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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2000-6-21
pubmed:abstractText
Transgenic mice bearing a transgene coding for a glucocorticoid receptor antisense mRNA, which partially blocks glucocorticoid receptor expression, were used in order to clarify the role of glucocorticoid receptors in the regulation of 5-HT(1A), 5-HT(1nonA) and 5-HT(2) binding sites labelled by quantitative autoradiography in the frontal and prefrontal cortex, striatum, hypothalamus, amygdala and raphe nuclei. We found that 1 nM [3H]8-hydroxy-2-[di-N-propylamino]tetralin ([3H]8-OH-DPAT) binding to 5-HT(1A) sites was decreased in strata oriens (-15.1+/-3.5%) and radiatum-lacunosum-moleculare (-13.3+/-4.3%) of the hippocampal CA(3) area, and 2 nM [3H]5-hydroxytryptamine binding to 5-HT(1nonA) sites in the presence of 100 nM 8-OH-DPAT and mesulergine was decreased in the dorsal subiculum (-17.8+/-6.9%). By contrast, 5-HT(2) sites labelled by 0.5 nM of (+/-)-1-(2, 5-dimethoxy-4-[125I]iodophenyl)-2-aminopropane was increased in the dorsal subiculum (+35.2+/-11.5%) and CA(2) area (+29.2+/-11.3%). The observed differences in binding to 5-HT(1) and 5-HT(2) sites were all located in areas of the hippocampus that contain both gluco- and mineralo-corticoid receptors, and no difference was observed in anatomical structures which contain only glucocorticoid receptors. Therefore, it seems that the important factor for the regulation of these 5-HT receptors is the interaction between gluco- and mineralo-corticoid receptors rather than the absolute density of glucocorticoid receptors. These results suggest that some of the alterations of the serotonergic neurotransmission observed in depressed patients might be secondary to an altered glucocorticoid receptor function.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
862
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
145-53
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:10799679-8-Hydroxy-2-(di-n-propylamino)tetralin, pubmed-meshheading:10799679-Amygdala, pubmed-meshheading:10799679-Animals, pubmed-meshheading:10799679-Autoradiography, pubmed-meshheading:10799679-Binding Sites, pubmed-meshheading:10799679-Brain Chemistry, pubmed-meshheading:10799679-Corpus Striatum, pubmed-meshheading:10799679-Down-Regulation, pubmed-meshheading:10799679-Hippocampus, pubmed-meshheading:10799679-Mice, pubmed-meshheading:10799679-Mice, Inbred Strains, pubmed-meshheading:10799679-Mice, Transgenic, pubmed-meshheading:10799679-Paraventricular Hypothalamic Nucleus, pubmed-meshheading:10799679-Prefrontal Cortex, pubmed-meshheading:10799679-RNA, Messenger, pubmed-meshheading:10799679-Raphe Nuclei, pubmed-meshheading:10799679-Receptor, Serotonin, 5-HT1B, pubmed-meshheading:10799679-Receptors, Glucocorticoid, pubmed-meshheading:10799679-Receptors, Serotonin, pubmed-meshheading:10799679-Receptors, Serotonin, 5-HT1, pubmed-meshheading:10799679-Serotonin Receptor Agonists, pubmed-meshheading:10799679-Tritium, pubmed-meshheading:10799679-Up-Regulation
pubmed:year
2000
pubmed:articleTitle
Central 5-HT(1) and 5-HT(2) binding sites in transgenic mice with reduced glucocorticoid receptor number.
pubmed:affiliation
INSERM U.501, Laboratoire d'Interactions Fonctionnelles en Neuroendocrinologie, IFR Jean-Roche, Université de la Méditerranée, UER de Médecine Nord, Boulevard Pierre Dramard, 13916, Marseille, France.
pubmed:publicationType
Journal Article