Linked Life Data

10792837

Source:http://linkedlifedata.com/resource/pubmed/id/10792837

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-6-29
pubmed:abstractText
The immunoglobulin (Ig)E immune response against protein antigens is profoundly influenced by the antigen dose used for immunization. Whereas immunization of CBA/J mice with low antigen doses results in the production of large amounts of IgE antibody, priming with high antigen doses leads to only marginal IgE antibody production in animals. However, in vitro restimulation of spleen cells from mice primed with high antigen doses leads to considerable activation of IgE-producing B cells, which suggests that B cells primed for IgE antibody production do exist among spleen cells. We investigated the modalities of activation of these memory B cells. The data presented here reveal that the anamnestic IgE immune response in vitro is strictly dependent on the presence of IgG1-expressing B cells, which differentiate after a sequential isotype switch into IgE-producing plasma cells with the help of primed CD4+ T cells. The induction of IgE-producing plasma cells requires a cognate interaction between B cells and CD4+ T cells. Interleukin (IL)-4 seems not to be involved in this process, since IgE production in vitro is resistant to suppression by anti-IL-4 monoclonal antibody. Finally, we show that IgG1-expressing B cells represent a relevant memory cell population in vivo also, but in contrast to the in vitro situation the final differentiation into IgE-producing plasma cells is dependent on IL-4.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0300-9475
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
461-71
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
The role of interleukin-4 in the regulation of sequential isotype switch from immunoglobulin G1 to immunoglobulin E antibody production.
pubmed:affiliation
Institute for Immunology, University of Münster, Münster, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't