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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2000-6-21
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pubmed:abstractText |
A real-time PCR assay for quantitation of Kaposi's sarcoma-associated herpes virus (KSHV or human herpesvirus 8) DNA was evaluated. The linear dynamic range was 10 to 10(5) copies of KSHV DNA (r(2) > 0.99). The accuracy of DNA purification and quantitation was less than +/-0.4 log(10) copies for samples that contained from 10 to 10(5) copies of KSHV DNA. Cell-associated KSHV DNA was quantitated over a range of infected cell frequencies from 0. 1 to 10(-5), and cell-free KSHV DNA in plasma was quantitated over a range of 100 to 10(6) copies/ml. Real-time PCR provides a convenient method for quantitation of cell-free and cell-associated KSHV DNA in laboratory and clinical specimens.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-10230356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-10400762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-7674745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-8523568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-8838705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-8908518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-9006369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-9029526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-9032391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-9535001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-9645637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-9736049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-9766492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-9894600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790138-9925538
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1992-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10790138-Cell Line,
pubmed-meshheading:10790138-DNA, Viral,
pubmed-meshheading:10790138-Herpesvirus 8, Human,
pubmed-meshheading:10790138-Humans,
pubmed-meshheading:10790138-Polymerase Chain Reaction,
pubmed-meshheading:10790138-Reproducibility of Results,
pubmed-meshheading:10790138-Sarcoma, Kaposi,
pubmed-meshheading:10790138-Sensitivity and Specificity,
pubmed-meshheading:10790138-Virion,
pubmed-meshheading:10790138-Virus Replication
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pubmed:year |
2000
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pubmed:articleTitle |
Quantitation of cell-free and cell-associated Kaposi's sarcoma-associated herpesvirus DNA by real-time PCR.
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pubmed:affiliation |
Division of Infectious Diseases, Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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