Linked Life Data

10786621

Source:http://linkedlifedata.com/resource/pubmed/id/10786621

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2000-5-24
pubmed:databankReference
pubmed:abstractText
The luciferase reporter gene system was used to assay the basal and Ara-C induced promoter activity of the human CR1 gene in K-562 erythroleukemia cells. Based on the results from clones of nested deletions, both basal and induced reporter gene activity fell to promoterless levels between constructs containing 79 bp (-79) and 41 bp (-41) upstream of the transcription start site. The -79 fragment was shifted in electrophoretic mobility assays using nuclear extracts from Ara-C induced and non-induced cells while the -41 bp fragment was not shifted. These data suggest that the 38 bp region between these constructs is necessary for the transcriptional activity of the CR1 gene and is involved in specifically binding transcription factors from the nuclei of induced and non-induced cells. Several potential transcription factor binding sites in this region were identified.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
1490
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
99-105
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Promoter activity of the 5' flanking region of the complement receptor type 1 (CR1) gene: basal and induced transcription.
pubmed:affiliation
Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, 87131-5276, USA.
pubmed:publicationType
Journal Article