Source:http://linkedlifedata.com/resource/pubmed/id/10781726
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2000-6-15
|
| pubmed:abstractText |
Akabane virus is a member of the genus Bunyavirus; it is pathogenic for ruminants and transmitted by arthropod vectors. Infection of adult cattle and sheep causes a transient viremia without obvious clinical signs, while infection of pregnant animals often causes fetal abnormalities including hydranencephaly, poliomyelitis and arthrogryposis. Infectious virus or viral antigens is present in the brain, spinal cord and skeletal muscle of infected fetuses. To understand the interaction between Akabane virus and bovine brain cells, we investigated the viral tropism using primary cultures of fetal bovine brain. The cultured neuronal cells, astroglia cells and microglia cells were distinguished by cell type specific antisera. Akabane virus was found to infect neuronal cells and astroglia cells, which led to degenerative death. No microglia cells were found infected. In some brain cultures, we observed different sensitivities of the cells to two Akabane virus strains: an attenuated strain infected and spread more readily than wild type virus. This difference was not observed in a hamster fibroblast cell line. Both viral and host determinants might be involved in the different susceptibility of brain cells to Akabane virus infection.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0378-1135
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
11
|
| pubmed:volume |
73
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
269-79
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10781726-Animals,
pubmed-meshheading:10781726-Antibodies, Monoclonal,
pubmed-meshheading:10781726-Antigens, Viral,
pubmed-meshheading:10781726-Astrocytes,
pubmed-meshheading:10781726-Brain Diseases,
pubmed-meshheading:10781726-Bunyaviridae Infections,
pubmed-meshheading:10781726-Cattle,
pubmed-meshheading:10781726-Cattle Diseases,
pubmed-meshheading:10781726-Cells, Cultured,
pubmed-meshheading:10781726-Cerebellum,
pubmed-meshheading:10781726-Cricetinae,
pubmed-meshheading:10781726-Culture Media, Serum-Free,
pubmed-meshheading:10781726-Cytopathogenic Effect, Viral,
pubmed-meshheading:10781726-Female,
pubmed-meshheading:10781726-Fetal Diseases,
pubmed-meshheading:10781726-Immunohistochemistry,
pubmed-meshheading:10781726-Microglia,
pubmed-meshheading:10781726-Microscopy, Fluorescence,
pubmed-meshheading:10781726-Neurons,
pubmed-meshheading:10781726-Pregnancy,
pubmed-meshheading:10781726-Simbu virus,
pubmed-meshheading:10781726-Tropism
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Preferential infection of neuronal and astroglia cells by Akabane virus in primary cultures of fetal bovine brain.
|
| pubmed:affiliation |
Laboratory of Immunogenetics, National Institute of Animal Health, Tsukuba, Ibaraki-ken, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|