Linked Life Data

10779803

Source:http://linkedlifedata.com/resource/pubmed/id/10779803

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2000-5-22
pubmed:abstractText
Polyclonal T cell lines specific for EBV proteins have proved efficient in preventing EBV-related immunoblastic lymphoma after allogeneic bone marrow transplantation. To gain insight into the composition of the EBV-specific T cell repertoire that ensured patient protection, we performed for the first time an extensive characterization of eight cytotoxic T cell lines selected in vitro against EBV-transformed autologous lymphoblastoid cell lines (BLCL). These T cell lines consist of 50-100 distinct T cell clones, of which 32-96% are specific for autologous BLCL. Moreover, we demonstrate that reactivities against only five EBV proteins (BZLF1, BMLF1, EBNA-3A, EBNA-3C, and LMP2) cover 86% (32/37) of the specificities detected. In addition, we describe an improved method of T cell harvesting using a CD25 selection procedure which reduces the time required to obtain specific T cells and improves the purity of EBV-specific T cells, thus showing promise for use in adoptive transfer protocols.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
164
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4924-32
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10779803-Adult, pubmed-meshheading:10779803-Animals, pubmed-meshheading:10779803-Antigens, Viral, pubmed-meshheading:10779803-Cell Line, Transformed, pubmed-meshheading:10779803-Cell Separation, pubmed-meshheading:10779803-Cell Transformation, Viral, pubmed-meshheading:10779803-Clone Cells, pubmed-meshheading:10779803-Coculture Techniques, pubmed-meshheading:10779803-Epitopes, T-Lymphocyte, pubmed-meshheading:10779803-Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor, pubmed-meshheading:10779803-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, pubmed-meshheading:10779803-Herpesvirus 4, Human, pubmed-meshheading:10779803-Humans, pubmed-meshheading:10779803-Immunophenotyping, pubmed-meshheading:10779803-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:10779803-Receptors, Interleukin-2, pubmed-meshheading:10779803-T-Lymphocyte Subsets
pubmed:year
2000
pubmed:articleTitle
The T cell repertoire selected in vitro against EBV: diversity, specificity, and improved purification through early IL-2 receptor alpha-chain (CD25)-positive selection.
pubmed:affiliation
Institut de Biologie, Institut National de la Santé et de la Recherche Médicale, Nantes, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't