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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2000-5-22
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pubmed:abstractText |
The genome of human herpes virus 8, which is associated with Kaposi's sarcoma, encodes proteins with similarities to cytokines and chemokines including a homologue of IL-6. Although the function of these viral proteins is unclear, they might have the potential to modulate the immune system. For viral IL-6 (vIL-6), it has been demonstrated that it stimulates IL-6-dependent cells, indicating that the IL-6R system is used. IL-6 binds to IL-6R, and the IL-6/IL-6R complex associates with gp130 which dimerizes and initiates intracellular signaling. Cells that only express gp130 but no IL-6R cannot be stimulated by IL-6 unless a soluble form of the IL-6R is present. This type of signaling has been shown for hematopoietic progenitor cells, endothelial cells, and smooth muscle cells. In this paper we show that purified recombinant vIL-6 binds to gp130 and stimulates primary human smooth muscle cells. IL-6R fails to bind vIL-6 and is not involved in its signaling. A Fc fusion protein of gp130 turned out to be a potent inhibitor of vIL-6. Our data demonstrate that vIL-6 is the first cytokine which directly binds and activates gp130. This property points to a possible role of this viral cytokine in the pathophysiology of human herpes virus 8.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokine Receptor gp130,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/IL6ST protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
164
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4672-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10779772-Aged,
pubmed-meshheading:10779772-Animals,
pubmed-meshheading:10779772-Antigens, CD,
pubmed-meshheading:10779772-COS Cells,
pubmed-meshheading:10779772-Chemical Precipitation,
pubmed-meshheading:10779772-Cloning, Molecular,
pubmed-meshheading:10779772-Cytokine Receptor gp130,
pubmed-meshheading:10779772-DNA-Binding Proteins,
pubmed-meshheading:10779772-Genetic Vectors,
pubmed-meshheading:10779772-Growth Inhibitors,
pubmed-meshheading:10779772-Humans,
pubmed-meshheading:10779772-Interleukin-6,
pubmed-meshheading:10779772-Male,
pubmed-meshheading:10779772-Membrane Glycoproteins,
pubmed-meshheading:10779772-Phosphorylation,
pubmed-meshheading:10779772-Protein Binding,
pubmed-meshheading:10779772-Receptors, Interleukin-6,
pubmed-meshheading:10779772-Recombinant Proteins,
pubmed-meshheading:10779772-STAT3 Transcription Factor,
pubmed-meshheading:10779772-Sarcoma, Kaposi,
pubmed-meshheading:10779772-Signal Transduction,
pubmed-meshheading:10779772-Trans-Activators,
pubmed-meshheading:10779772-Tumor Cells, Cultured,
pubmed-meshheading:10779772-Viral Proteins
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pubmed:year |
2000
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pubmed:articleTitle |
IL-6 receptor independent stimulation of human gp130 by viral IL-6.
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pubmed:affiliation |
I. Medizinische Klinik, Abteilung Pathophysiologie, Johannes Gutenberg-Universität Mainz, Mainz, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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