Linked Life Data

10775376

Source:http://linkedlifedata.com/resource/pubmed/id/10775376

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-6-19
pubmed:abstractText
In-line connected electrochemical (EC) and diode array (DAD) detectors were compared in the reversed-phase high-performance liquid chromatographic (RP-HPLC) analysis of coenzymes Q(9) and Q(10) in some food materials (beef steak, beef heart, Baltic herring fillet, and rye flour). Coenzymes Q(9) and Q(10) were extracted from the samples using a 5:1 n-hexane-ethanol mixture. Coefficient of variation (CV%) of quadruplicate or quintuplicate determined samples for coenzymes Q(9) and Q(10) was <10 by both EC detector and DAD. Responses of the detection systems were linear in the range evaluated, 10-200 ng/injection, and had correlation coefficients exceeding 0.999. Recoveries of added coenzymes Q(9) and Q(10) varied 73-105% for DAD and 74-103% for EC detector, respectively. Detection limits for coenzymes Q(9) and Q(10) using the DAD system were 4 and 6 ng/injection, respectively, and 0.2 and 0.3 ng/injection by EC detection. Results derived from the two detection systems were generally similar. However, although EC detector was 20-fold more sensitive, the selectivity was, in some cases, poorer than that of DAD.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-8561
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1229-33
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Comparison of in-line connected diode array and electrochemical detectors in the high-performance liquid chromatographic analysis of coenzymes Q(9) and Q(10) in food materials.
pubmed:affiliation
Agricultural Research Centre of Finland, Food Research, L-talo, 31600 Jokioinen, Finland. pirjo.mattila@mtt.fi
pubmed:publicationType
Journal Article