Source:http://linkedlifedata.com/resource/pubmed/id/10754293
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
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| pubmed:dateCreated |
2000-5-9
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| pubmed:abstractText |
Optimal CD4+ T cell activation requires the cooperation of multiple signaling pathways coupled to the TCR-CD3 complex and to the CD28 costimulatory molecule. In this study, we have investigated the expression of surface CXC chemokine receptor 4 (CXCR4) in enriched populations of CD4+ T PBL, stimulated with anti-CD3 and anti-CD28 mAbs, immobilized on plastic. Anti-CD3 alone induced a progressive down-regulation of surface CXCR4, accompanied by a significant decline in the entry of the HXB2 T cell line-tropic (X4-tropic) HIV-1 clone in CD4+ T cells. Of note, this effect was strictly dependent on the presence in culture of CD14+ monocytes. On the other hand, anti-CD28 alone induced a small but reproducible increase in the expression of surface CXCR4 as well as in the entry of HXB2 HIV-1 clone in resting CD4+ T cells. When the two mAbs were used in combination, anti-CD28 potently synergized with anti-CD3 in inducing the expression of CD69 activation marker and stimulating the proliferation of CD4+ T cells. On the other hand, anti-CD28 counteracted the CXCR4 down-modulation induced by anti-CD3. The latter effect was particularly evident when anti-CD28 was associated to suboptimal concentrations of anti-CD3. Because CXCR4 is the major coreceptor for the highly cytopathic X4-tropic HIV-1 strains, which preferentially replicate in proliferating CD4+ T cells, the ability of anti-CD28 to up-regulate the surface expression of CXCR4 in both resting and activated CD4+ T cells provides one relevant mechanism for the progression of HIV-1 disease.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD14,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CCR5,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CXCR4,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
|
| pubmed:volume |
164
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
4018-24
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10754293-Adult,
pubmed-meshheading:10754293-Antibodies, Monoclonal,
pubmed-meshheading:10754293-Antigens, CD14,
pubmed-meshheading:10754293-Antigens, CD28,
pubmed-meshheading:10754293-Antigens, CD3,
pubmed-meshheading:10754293-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10754293-Cell Membrane,
pubmed-meshheading:10754293-Cells, Cultured,
pubmed-meshheading:10754293-Dose-Response Relationship, Immunologic,
pubmed-meshheading:10754293-Down-Regulation,
pubmed-meshheading:10754293-Extracellular Space,
pubmed-meshheading:10754293-Gene Products, tat,
pubmed-meshheading:10754293-HIV-1,
pubmed-meshheading:10754293-Humans,
pubmed-meshheading:10754293-Interphase,
pubmed-meshheading:10754293-Monocytes,
pubmed-meshheading:10754293-Receptors, CCR5,
pubmed-meshheading:10754293-Receptors, CXCR4,
pubmed-meshheading:10754293-tat Gene Products, Human Immunodeficiency Virus
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| pubmed:year |
2000
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| pubmed:articleTitle |
Engagement of CD28 modulates CXC chemokine receptor 4 surface expression in both resting and CD3-stimulated CD4+ T cells.
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| pubmed:affiliation |
Institute of Human Virology, University of Maryland, Baltimore, MD 21201, USA. secchier@umbi.umd.edu
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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