Linked Life Data

10753059

Source:http://linkedlifedata.com/resource/pubmed/id/10753059

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pubmed-article:10753059pubmed:abstractTextTwo monoclonal antibodies (mAbs) specific for the human parainfluenza virus type 2 (hPIV-2) V protein were obtained by immunizing mice with the V protein recombinantly expressed in Escherichia coli. Both mAbs were found to react with the V protein in ELISA and in Western blot analysis. Using these mAbs and previously obtained mAbs specific for hPIV-2 nucleoprotein (NP) or hPIV-2 phospho-(P) protein, we examined the intracellular distributions of the V, P and NP proteins in hPIV-2-infected cells by indirect immunofluorescence analyses. The P and NP proteins were organized in numerous granules in the cytoplasm of hPIV-2 infected cells. In contrast, the V protein showed diffuse nuclear and cytoplasm distributions.lld:pubmed
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pubmed-article:10753059pubmed:articleTitleIsolation of monoclonal antibodies directed against the V protein of human parainfluenza virus type 2 and localization of the V protein in virus-infected cells.lld:pubmed
pubmed-article:10753059pubmed:affiliationDepartment of Microbiology, Mie University School of Medicine, Japan. nishio@doc.medic.mie-u.ac.jplld:pubmed
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