|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2000-5-2
|
|
pubmed:abstractText |
Two monoclonal antibodies (mAbs) specific for the human parainfluenza virus type 2 (hPIV-2) V protein were obtained by immunizing mice with the V protein recombinantly expressed in Escherichia coli. Both mAbs were found to react with the V protein in ELISA and in Western blot analysis. Using these mAbs and previously obtained mAbs specific for hPIV-2 nucleoprotein (NP) or hPIV-2 phospho-(P) protein, we examined the intracellular distributions of the V, P and NP proteins in hPIV-2-infected cells by indirect immunofluorescence analyses. The P and NP proteins were organized in numerous granules in the cytoplasm of hPIV-2 infected cells. In contrast, the V protein showed diffuse nuclear and cytoplasm distributions.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/V protein, human parainfluenza...,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Structural Proteins
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0300-8584
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
188
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
79-82
|
|
pubmed:dateRevised |
2007-2-21
|
|
pubmed:meshHeading |
pubmed-meshheading:10753059-Animals,
pubmed-meshheading:10753059-Antibodies, Monoclonal,
pubmed-meshheading:10753059-Antibodies, Viral,
pubmed-meshheading:10753059-Blotting, Western,
pubmed-meshheading:10753059-Cell Nucleus,
pubmed-meshheading:10753059-Cercopithecus aethiops,
pubmed-meshheading:10753059-Cytoplasm,
pubmed-meshheading:10753059-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10753059-HeLa Cells,
pubmed-meshheading:10753059-Humans,
pubmed-meshheading:10753059-Mice,
pubmed-meshheading:10753059-Microscopy, Fluorescence,
pubmed-meshheading:10753059-Nucleoproteins,
pubmed-meshheading:10753059-Parainfluenza Virus 2, Human,
pubmed-meshheading:10753059-Phosphoproteins,
pubmed-meshheading:10753059-Recombinant Fusion Proteins,
pubmed-meshheading:10753059-Vero Cells,
pubmed-meshheading:10753059-Viral Proteins,
pubmed-meshheading:10753059-Viral Structural Proteins
|
|
pubmed:year |
1999
|
|
pubmed:articleTitle |
Isolation of monoclonal antibodies directed against the V protein of human parainfluenza virus type 2 and localization of the V protein in virus-infected cells.
|
|
pubmed:affiliation |
Department of Microbiology, Mie University School of Medicine, Japan. nishio@doc.medic.mie-u.ac.jp
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
