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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-6-1
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pubmed:abstractText |
Overexpression of wild-type p53 in cancer cells by adenovirus-mediated p53 gene transfer can result in the induction of apoptosis. To identify the potential mediators of this p53-induced apoptosis, we examined apoptotic protein levels in human lung cancer cells after Adp53 gene transfer. We observed up-regulation of Bax and Bak protein levels 18-36 h after transduction with Adp53 in H1299, H358, and H322 lung cancer cells. Contrary to expected observations, no changes in Bcl-2 and Bcl-X(L) protein levels were observed. Morphological cell changes and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling staining showed evidence of apoptosis in all cell lines 48 h after transduction with Adp53. These results indicate that the induction of apoptosis by adenovirus-mediated p53 transfer may be mediated by the induction of proapoptotic mechanisms rather than suppression of antiapoptotic mechanisms.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BAK1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/BAX protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/BCL2L1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Recombinant,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2 Homologous Antagonist-Killer...,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-X Protein
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1078-0432
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
887-90
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10741712-Adenoviridae,
pubmed-meshheading:10741712-Apoptosis,
pubmed-meshheading:10741712-Blotting, Western,
pubmed-meshheading:10741712-DNA, Recombinant,
pubmed-meshheading:10741712-Gene Transfer Techniques,
pubmed-meshheading:10741712-Humans,
pubmed-meshheading:10741712-Lung Neoplasms,
pubmed-meshheading:10741712-Membrane Proteins,
pubmed-meshheading:10741712-Proto-Oncogene Proteins,
pubmed-meshheading:10741712-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:10741712-Tumor Cells, Cultured,
pubmed-meshheading:10741712-Tumor Suppressor Protein p53,
pubmed-meshheading:10741712-Up-Regulation,
pubmed-meshheading:10741712-bcl-2 Homologous Antagonist-Killer Protein,
pubmed-meshheading:10741712-bcl-2-Associated X Protein,
pubmed-meshheading:10741712-bcl-X Protein
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pubmed:year |
2000
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pubmed:articleTitle |
Up-regulation of the proapoptotic mediators Bax and Bak after adenovirus-mediated p53 gene transfer in lung cancer cells.
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pubmed:affiliation |
Department of Surgical Oncology, The University of Texas M.D Anderson Cancer Center, Houston 77030, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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