Linked Life Data

10734195

Source:http://linkedlifedata.com/resource/pubmed/id/10734195

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2000-6-6
pubmed:databankReference
pubmed:abstractText
Magnesium ions play important roles in the structure and function of nucleic acids. Whereas the tertiary folding of RNA often requires magnesium ions binding to tight places where phosphates are clustered, the molecular basis of the interactions of magnesium ions with RNA helical regions is less well understood. We have refined the crystal structures of four decamer oligonucleotides, d(ACCGGCCGGT), r(GCG)d(TATACGC), r(GC)d(GTATACGC) and r(G)d(GCGTATACGC) with bound hexahydrated magnesium ions at high resolution. The structures reveal that A-form nucleic acid has characteristic [Mg(H(2)O)(6)](2+)binding modes. One mode has the ion binding in the deep major groove of a GpN step at the O6/N7 sites of guanine bases via hydrogen bonds. Our crystallographic observations are consistent with the recent NMR observations that in solution [Co(NH(3))(6)](3+), a model ion of [Mg(H(2)O)(6)](2+), binds in an identical manner. The other mode involves the binding of the ion to phosphates, bridging across the outer mouth of the narrow major groove. These [Mg(H(2)O)(6)](2+)ions are found at the most negative electrostatic potential regions of A-form duplexes. We propose that these two binding modes are important in the global charge neutralization, and therefore stability, of A-form duplexes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-10334337, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-10542099, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-10555960, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-2303044, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-2714286, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-343112, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-3978072, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-7044422, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-7301586, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-7681688, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-7690496, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-7719853, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-8371984, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-8604309, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-8679609, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-9195889, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-9393863, http://linkedlifedata.com/resource/pubmed/commentcorrection/10734195-9622486
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1362-4962
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1760-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Hexahydrated magnesium ions bind in the deep major groove and at the outer mouth of A-form nucleic acid duplexes.
pubmed:affiliation
Bl07 CLSL, Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, 601 South Goodwin Avenue, Urbana, IL 61801, USA,
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.