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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-5-11
pubmed:databankReference
pubmed:abstractText
The four known members of the KCND/Kv4 channel family encode voltage-gated potassium channels. Recent studies provide evidence that members of the Kv4 channel family are responsible for native, rapidly inactivating (A-type) currents described in heart (I(TO)) and neurons (I(SA)). In this study, we cloned the human KCND1 cDNA, localized the KCND1 gene to chromosome Xp11.23-p11.3, and determined the genomic structure and tissue-specific expression of the KCND1, KCND2, and KCND3 genes, respectively. The open reading frame of Kv4. 1 is 1941 nucleotides long, predicting a protein of 647 amino acids. The deduced protein sequence of Kv4.1 shows an overall identity of 60% with Kv4.2 and Kv4.3L and corresponds to the common structure of voltage-gated potassium channels. KCND1-specific transcripts were detectable in human brain, heart, liver, kidney, thyroid gland, and pancreas, as revealed by Northern blot and RT-PCR experiments. The comparison of the expression patterns of the known Kv4 family members shows subtype specificity with significant overlaps. The KCND gene structures exhibit an evolutionarily conserved exon pattern with a large first exon containing the intracellular N-terminus and the putative membrane-spanning regions S1 to S5, as well as part of the pore region. The KCND3 gene contains an additional exon of 57 bp, which is not present in the other two KCND genes and gives rise to the C-terminal splice KCND3L variant with an insertion of 19 amino acids.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0888-7543
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Academic Press.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
144-54
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10729221-Chromosome Mapping, pubmed-meshheading:10729221-Cloning, Molecular, pubmed-meshheading:10729221-DNA, Complementary, pubmed-meshheading:10729221-Electric Conductivity, pubmed-meshheading:10729221-Exons, pubmed-meshheading:10729221-Gene Expression, pubmed-meshheading:10729221-Gene Library, pubmed-meshheading:10729221-Genome, pubmed-meshheading:10729221-Humans, pubmed-meshheading:10729221-Introns, pubmed-meshheading:10729221-Ion Channel Gating, pubmed-meshheading:10729221-Molecular Sequence Data, pubmed-meshheading:10729221-Polymerase Chain Reaction, pubmed-meshheading:10729221-Potassium Channels, pubmed-meshheading:10729221-Potassium Channels, Voltage-Gated, pubmed-meshheading:10729221-RNA, Messenger, pubmed-meshheading:10729221-Sequence Alignment, pubmed-meshheading:10729221-Sequence Homology, Amino Acid, pubmed-meshheading:10729221-Shal Potassium Channels, pubmed-meshheading:10729221-Tissue Distribution, pubmed-meshheading:10729221-Transcription, Genetic
pubmed:year
2000
pubmed:articleTitle
Gene structures and expression profiles of three human KCND (Kv4) potassium channels mediating A-type currents I(TO) and I(SA).
pubmed:affiliation
Institute of Neural Signal Transduction, Centre for Molecular Neurobiology, Martinistrasse 52, Hamburg, 20246, Germany. isbrandt@uni-hamburg.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't