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rdf:type |
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lifeskim:mentions |
umls-concept:C0006669,
umls-concept:C0011306,
umls-concept:C0017262,
umls-concept:C0019764,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0332307,
umls-concept:C0439640,
umls-concept:C0547047,
umls-concept:C0871261,
umls-concept:C1413243,
umls-concept:C1424497,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911684,
umls-concept:C2911692
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pubmed:issue |
7
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pubmed:dateCreated |
2000-5-4
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pubmed:abstractText |
These studies were performed to establish whether functional receptors for calcitonin gene-related peptide (CGRP) are present on human dendritic cells (DCs) and to investigate potential immunomodulatory effects of CGRP on DCs other than Langerhans cells. Reverse transcriptase-PCR revealed expression of mRNA for a type 1 CGRP receptor by mature and immature blood-derived DCs. Sequence analysis confirmed the identity of the type 1 CGRP receptor (CGRP-R1). Addition of CGRP (10-7 M) to mature and immature DCs resulted in mobilization of intracellular calcium. Treatment of immature DCs with CGRP (10-7 M), before and after maturation in monocyte-conditioned medium, resulted in decreased cell surface expression of HLA-DR MHC class II and the costimulatory molecule, CD86. Treatment of immature DCs with CGRP (10-7 M) also resulted in decreased expression of CD86, but expression of HLA-DR was unchanged. When CGRP-treated mature DCs were used to stimulate allogeneic T cells, proliferative responses were dampened (approximately 50%), especially at low DC:T cell ratios (1:360). This effect was not observed with CGRP-treated, immature DCs. In contrast, CGRP-treated mature or immature DCs were no less efficient than untreated DCs in driving syngeneic T cell-proliferative responses to staphylococcal enterotoxin B. We conclude that mature and immature DCs express type 1 CGRP receptors and that signaling through these receptors may dampen mature DC-driven T cell proliferation most likely via down-regulation of CD86 and HLA-DR.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/CD86 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcitonin Gene-Related Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Enterotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Immunosuppressive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Isoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Calcitonin Gene-Related...,
http://linkedlifedata.com/resource/pubmed/chemical/Superantigens,
http://linkedlifedata.com/resource/pubmed/chemical/enterotoxin B, staphylococcal
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
164
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3494-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10725702-Antigens, CD,
pubmed-meshheading:10725702-Antigens, CD86,
pubmed-meshheading:10725702-Calcitonin Gene-Related Peptide,
pubmed-meshheading:10725702-Calcium,
pubmed-meshheading:10725702-Cell Differentiation,
pubmed-meshheading:10725702-Dendritic Cells,
pubmed-meshheading:10725702-Enterotoxins,
pubmed-meshheading:10725702-HLA-DR Antigens,
pubmed-meshheading:10725702-Humans,
pubmed-meshheading:10725702-Immunosuppressive Agents,
pubmed-meshheading:10725702-Isoantigens,
pubmed-meshheading:10725702-Lymphocyte Activation,
pubmed-meshheading:10725702-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:10725702-Membrane Glycoproteins,
pubmed-meshheading:10725702-RNA, Messenger,
pubmed-meshheading:10725702-Receptors, Calcitonin Gene-Related Peptide,
pubmed-meshheading:10725702-Staphylococcus aureus,
pubmed-meshheading:10725702-Superantigens,
pubmed-meshheading:10725702-T-Lymphocytes
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pubmed:year |
2000
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pubmed:articleTitle |
Calcitonin gene-related peptide decreases expression of HLA-DR and CD86 by human dendritic cells and dampens dendritic cell-driven T cell-proliferative responses via the type I calcitonin gene-related peptide receptor.
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pubmed:affiliation |
Laboratories of Cellular Physiology Rockefeller University, New York, NY 10021, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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