Source:http://linkedlifedata.com/resource/pubmed/id/10709107
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2000-3-23
|
| pubmed:abstractText |
Integrin-mediated adhesion of cells to extracellular matrix proteins has been shown to activate various intracellular signaling events. In the present study, we demonstrate that the addition of a monoclonal antibody raised against the beta4 integrin subunit in the culture medium of a clone derived from the colon adenocarcinoma cell line LoVo specifically results in stimulation of cell migration and invasion through reconstituted basement membrane matrices. Moreover, an increase in MMP-2 activity is observed. Conversely, monoclonal anti-alpha6 and anti-beta1 have no effect on MMP-2 expression. The s. c. co-injection of adenocarcinoma cells with antibodies raised against the beta4 integrin subunit to immunosuppressed newborn rats gives rise to tumors displaying altered and disorganized peri-tumoral basement membranes compared with tumors obtained when cells are injected with adenocarcinoma cells alone. Higher metastatic capacity of cells results when they are co-injected with antibodies to the beta4 integrin subunit. Our results suggest that the beta4 subunit of alpha6beta4 integrin, a laminin receptor in colon adenocarcinoma, may be responsible for the specific signals which stimulate cell motility, expression of MMP-2 and tumor invasion.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin beta4,
http://linkedlifedata.com/resource/pubmed/chemical/Laminin,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/laminin 1
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
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| pubmed:issn |
0020-7136
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2000 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:day |
15
|
| pubmed:volume |
85
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
850-6
|
| pubmed:dateRevised |
2007-7-24
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| pubmed:meshHeading |
pubmed-meshheading:10709107-Adenocarcinoma,
pubmed-meshheading:10709107-Animals,
pubmed-meshheading:10709107-Animals, Newborn,
pubmed-meshheading:10709107-Antibodies, Monoclonal,
pubmed-meshheading:10709107-Antigens, CD,
pubmed-meshheading:10709107-Blotting, Southern,
pubmed-meshheading:10709107-Cell Movement,
pubmed-meshheading:10709107-Colonic Neoplasms,
pubmed-meshheading:10709107-Humans,
pubmed-meshheading:10709107-Immunohistochemistry,
pubmed-meshheading:10709107-Integrin beta4,
pubmed-meshheading:10709107-Laminin,
pubmed-meshheading:10709107-Matrix Metalloproteinase 2,
pubmed-meshheading:10709107-Microscopy, Electron,
pubmed-meshheading:10709107-Neoplasm Invasiveness,
pubmed-meshheading:10709107-Rats,
pubmed-meshheading:10709107-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10709107-Tumor Cells, Cultured
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Anti-beta4 integrin antibodies enhance migratory and invasive abilities of human colon adenocarcinoma cells and their MMP-2 expression.
|
| pubmed:affiliation |
INSERM U 45, Hôpital Edouard Herriot, Lyon, France.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|