| pubmed-article:10706782 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10706782 | lifeskim:mentions | umls-concept:C0017906 | lld:lifeskim |
| pubmed-article:10706782 | lifeskim:mentions | umls-concept:C0547040 | lld:lifeskim |
| pubmed-article:10706782 | lifeskim:mentions | umls-concept:C0008550 | lld:lifeskim |
| pubmed-article:10706782 | lifeskim:mentions | umls-concept:C1881695 | lld:lifeskim |
| pubmed-article:10706782 | lifeskim:mentions | umls-concept:C1709915 | lld:lifeskim |
| pubmed-article:10706782 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10706782 | pubmed:dateCreated | 2000-5-9 | lld:pubmed |
| pubmed-article:10706782 | pubmed:abstractText | An original, unambiguous microassay of galactofuranose (Galf) residues in glycoconjugates is described. The method involves mild acid methanolysis (5 mM HCl) for 3 h at 84 degrees C followed by high pH anion-exchange chromatography using a routine monosaccharide system. The methanolysis products Mealpha-Galf and Mebeta-Galf were characterized chromatographically by comparison with the authentic compounds and by their response to treatment with mild acid and with beta-galactofuranosidase. Testing against p-nitrophenyl-beta-Galf and UDPalpha-Galf showed the method to be applicable to both alpha- and beta- galactofuranosides over the range 10-200 pmol. The results of partial mild methanolysis over shorter periods were consistent with initial inversion of anomeric configuration at methylation followed by anomerization to an equilibrium mixture of alpha- and beta-forms. When applied to a sample of invertase from Aspergillus nidulans, the method indicated that all of the mild acid-labile galactose (78% of the total galactose present) was in the form of a galactofuranoside and that much of this was in the beta-configuration. As expected, when applied to asialofetuin (known to contain galactose only in the pyranoside form, Galp), NPalpha-Galp, NPbeta-Galp, or UDPalpha-Galp, mild acid methanolysis failed to produce any galactofuranoside. | lld:pubmed |
| pubmed-article:10706782 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10706782 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10706782 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10706782 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10706782 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10706782 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10706782 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10706782 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10706782 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10706782 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10706782 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10706782 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:10706782 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:10706782 | pubmed:author | pubmed-author:PeberdyJ FJF | lld:pubmed |
| pubmed-article:10706782 | pubmed:author | pubmed-author:HemmingF WFW | lld:pubmed |
| pubmed-article:10706782 | pubmed:author | pubmed-author:WallisG LGL | lld:pubmed |
| pubmed-article:10706782 | pubmed:copyrightInfo | Copyright 2000 Academic Press. | lld:pubmed |
| pubmed-article:10706782 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10706782 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:10706782 | pubmed:volume | 279 | lld:pubmed |
| pubmed-article:10706782 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10706782 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10706782 | pubmed:pagination | 136-41 | lld:pubmed |
| pubmed-article:10706782 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:10706782 | pubmed:meshHeading | pubmed-meshheading:10706782... | lld:pubmed |
| pubmed-article:10706782 | pubmed:meshHeading | pubmed-meshheading:10706782... | lld:pubmed |
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| pubmed-article:10706782 | pubmed:meshHeading | pubmed-meshheading:10706782... | lld:pubmed |
| pubmed-article:10706782 | pubmed:meshHeading | pubmed-meshheading:10706782... | lld:pubmed |
| pubmed-article:10706782 | pubmed:meshHeading | pubmed-meshheading:10706782... | lld:pubmed |
| pubmed-article:10706782 | pubmed:meshHeading | pubmed-meshheading:10706782... | lld:pubmed |
| pubmed-article:10706782 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10706782 | pubmed:articleTitle | An unambiguous microassay of galactofuranose residues in glycoconjugates using mild methanolysis and high pH anion-exchange chromatography. | lld:pubmed |
| pubmed-article:10706782 | pubmed:affiliation | School of Biomedical Sciences, Medical School, University of Nottingham, Nottingham, NG7 2UH, United Kingdom. Frank.Hemming@nottingham.ac.uk | lld:pubmed |
| pubmed-article:10706782 | pubmed:publicationType | Journal Article | lld:pubmed |
