|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2000-4-24
|
|
pubmed:abstractText |
Application of flow cytometry (FCM) to microbial analysis of milk is hampered by the presence of milk proteins and lipid particles. Here we report on the development of a rapid (</=1-h) FCM assay based on enzymatic clearing of milk to determine total bacteria in milk. When bacteria were added to ultra-heat-treated milk, a good correlation (r >/= 0.98) between the FCM assay and the more conventional methods of plating and direct microscopic counting was achieved. Raw milk data showed a significant correlation (P < 0.01) and a good agreement (r = 0.91) between FCM and standard plate count methods. The detection limit of the FCM assay was </=10(4) bacteria ml of milk(-1). This limit is below the level of detection required to satisfy legislation in many countries and states.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-12514070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-3096202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-7533254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-7535888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-7766074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-7799425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-7811064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-7989272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-8220231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-8227633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-8227634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-8227635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-8227636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-8987359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-9161919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-9351209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10698799-9633655
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0099-2240
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
66
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1228-32
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
A flow cytometry method for rapid detection and enumeration of total bacteria in milk.
|
|
pubmed:affiliation |
Centre for Fluorimetric Applications in Biotechnology, Department of Biological Sciences, Macquarie University, Sydney, New South Wales 2109, Australia. tgunasek@rna.bio.mq.edu.au
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
