Linked Life Data

10696068

Source:http://linkedlifedata.com/resource/pubmed/id/10696068

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-4-14
pubmed:abstractText
The urokinase receptor (uPAR) binds and localizes urokinase activity at cellular surfaces, facilitating fibrinolysis and cellular migration at sites of tissue injury. uPAR also participates in cellular signaling and regulates integrin-dependent adhesion and migration in vitro. We now report evidence that uPAR occupancy regulates cellular migration in vivo in the absence of functional urokinase. Recombinant murine KC (1.5 microg), a potent neutrophil chemoattractant, was delivered to the lungs of wild-type, urokinase-deficient or uPAR-deficient mice 18 h after intraperitoneal injection of 200 microg human immunoglobulin G (IgG) or a fusion protein composed of an amino-terminal receptor-binding fragment of urokinase and a human IgG Fc fragment (GFD-Fc). Whole lung lavage for recovery of leukocytes was performed 4 h later. KC treatment resulted in a 100-fold increase in lavage neutrophils. GFD-Fc injection resulted in >50% reduction in neutrophil influx in both wild-type and urokinase-deficient animals but had no effect on uPAR -/- mice. A concomitant reduction in alveolar protein leakage but no change in numbers of circulating neutrophils accompanied this attenuated inflammatory response. The reduction in neutrophil influx induced by GFD-Fc is thus related to uPAR occupancy and yet not due to disruption of uPAR-mediated proteolysis. These observations verify that protease-independent functions of uPAR operate in vivo and identify uPAR as a potential target for regulation of inflammatory processes characterized by neutrophil-mediated injury.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1044-1549
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
316-22
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10696068-Administration, Intranasal, pubmed-meshheading:10696068-Animals, pubmed-meshheading:10696068-Cell Adhesion, pubmed-meshheading:10696068-Cell Movement, pubmed-meshheading:10696068-Coloring Agents, pubmed-meshheading:10696068-Evans Blue, pubmed-meshheading:10696068-Humans, pubmed-meshheading:10696068-Immunoglobulin Constant Regions, pubmed-meshheading:10696068-Immunoglobulin G, pubmed-meshheading:10696068-Mice, pubmed-meshheading:10696068-Mice, Inbred C57BL, pubmed-meshheading:10696068-Mice, Transgenic, pubmed-meshheading:10696068-Neutrophils, pubmed-meshheading:10696068-Pulmonary Alveoli, pubmed-meshheading:10696068-Receptors, Cell Surface, pubmed-meshheading:10696068-Receptors, Urokinase Plasminogen Activator, pubmed-meshheading:10696068-Recombinant Proteins, pubmed-meshheading:10696068-Signal Transduction, pubmed-meshheading:10696068-Urokinase-Type Plasminogen Activator
pubmed:year
2000
pubmed:articleTitle
Nonproteolytic role for the urokinase receptor in cellular migration in vivo.
pubmed:affiliation
Department of Medicine, Children's Hospital, Boston, Massachusetts 02115, USA. waltz@a1.tch.harvard.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.