Linked Life Data

10695929

Source:http://linkedlifedata.com/resource/pubmed/id/10695929

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-3-14
pubmed:abstractText
Male weanling Wistar rats (n = 15), weighing 200-220 g, were allocated for 6 wk to diets containing 1% (by weight) of conjugated linoleic acid (CLA), either as the 9c,11 t-isomer, the 10t,12c-isomer, or as a mixture containing 45% of each of these isomers. The five rats of the control group received 1% of oleic acid instead. Selected enzyme activities were determined in different tissues after cellular subfractionation. None of the CLA-diet induced a hepatic peroxisome-proliferation response, as evidenced by a lack of change in the activity of some characteristic enzymes [i.e., acyl-CoA oxidase, CYP4A1, but also carnitine palmitoyltransferase-I (CPT-I)] or enzyme affected by peroxisome-proliferators (glutathione S-transferase). In addition to the liver, the activity of the rate-limiting beta-oxidation enzyme in mitochondria, CPT-I, did not change either in skeletal muscle or in heart. Conversely, its activity increased more than 30% in the control value in epididymal adipose tissue of the animals fed the CLA-diets containing the 10t,12c-isomer. Conversely, the activity of phosphatidate phosphohydrolase, a rate-limiting enzyme in glycerolipid neosynthesis, remained unchanged in adipose tissue. Kinetic studies conducted on hepatic CPT-I and peroxisomal acyl-CoA oxidase with CoA derivatives predicted a different channeling of CLA isomers through the mitochondrial or the peroxisomal oxidation pathways. In conclusion, the 10t,12c-CLA isomer seems to be more efficiently utilized by the cells than its 9c,11t homolog, though the Wistar rat species appeared to be poorly responsive to CLA diets for the effects measured.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0024-4201
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
91-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10695929-Acyl-CoA Oxidase, pubmed-meshheading:10695929-Adipose Tissue, pubmed-meshheading:10695929-Alkane 1-Monooxygenase, pubmed-meshheading:10695929-Animals, pubmed-meshheading:10695929-Carnitine O-Palmitoyltransferase, pubmed-meshheading:10695929-Cytochrome P-450 Enzyme System, pubmed-meshheading:10695929-Enzymes, pubmed-meshheading:10695929-Glutathione Transferase, pubmed-meshheading:10695929-Kinetics, pubmed-meshheading:10695929-Linoleic Acid, pubmed-meshheading:10695929-Lipid Metabolism, pubmed-meshheading:10695929-Liver, pubmed-meshheading:10695929-Male, pubmed-meshheading:10695929-Mitochondria, pubmed-meshheading:10695929-Mixed Function Oxygenases, pubmed-meshheading:10695929-Muscle, Skeletal, pubmed-meshheading:10695929-Myocardium, pubmed-meshheading:10695929-Oxidoreductases, pubmed-meshheading:10695929-Rats, pubmed-meshheading:10695929-Rats, Wistar
pubmed:year
2000
pubmed:articleTitle
Effects of conjugated linoleic acid isomers on lipid-metabolizing enzymes in male rats.
pubmed:affiliation
I.N.R.A, Unité de Nutrition Lipidique, Dijon, France. jean-charles.martin@ibaic.u-psud.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't