|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2000-3-31
|
|
pubmed:abstractText |
Multipotential mesenchymal stem cells capable of chondro-osseous induction contribute to the endochondral callus of healing fractured bone. Microvascular pericytes serving the role of multipotential mesenchymal stem cells are considered osteoprogenitors because they express type I collagen, alkaline phosphatase enzyme activity, osteocalcin immunoreactivity, and bone sialoprotein mRNA. Previous electron microscopic studies indicate that this cell type has a contribution to the fracture callus. Limited data suggest that pericytes may also assume a chondrogenic phenotype. We undertook in vitro studies to understand how the chondro-osseous phenotype of the pericyte might be regulated. Using Northern analysis and semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR), we found that cultured pericytes produce aggrecan and type II collagen mRNA indicating their chondrogenic potential. Aggrecan message is elevated by BMP-2 as analyzed by both Northern hybridization and RT-PCR. This finding suggests a regulatory role for this morphogen on this phenotype in pericytes. RT-PCR amplified versican product was also associated with pericyte cultures but was not affected by BMP-2. Our data strongly support a chondrogenic role for the pericyte and that the phenotype is regulated at least in part by BMP.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aggrecans,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 2,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chondroitin Sulfate Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Versicans
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0006-291X
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright 2000 Academic Press.
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
5
|
|
pubmed:volume |
269
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
172-8
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:10694496-Aggrecans,
pubmed-meshheading:10694496-Animals,
pubmed-meshheading:10694496-Base Sequence,
pubmed-meshheading:10694496-Bone Morphogenetic Protein 2,
pubmed-meshheading:10694496-Bone Morphogenetic Proteins,
pubmed-meshheading:10694496-Cattle,
pubmed-meshheading:10694496-Cells, Cultured,
pubmed-meshheading:10694496-Chondrogenesis,
pubmed-meshheading:10694496-Chondroitin Sulfate Proteoglycans,
pubmed-meshheading:10694496-Collagen,
pubmed-meshheading:10694496-DNA Primers,
pubmed-meshheading:10694496-Extracellular Matrix Proteins,
pubmed-meshheading:10694496-Fracture Healing,
pubmed-meshheading:10694496-Gene Expression,
pubmed-meshheading:10694496-Lectins, C-Type,
pubmed-meshheading:10694496-Microcirculation,
pubmed-meshheading:10694496-Pericytes,
pubmed-meshheading:10694496-Phenotype,
pubmed-meshheading:10694496-Proteoglycans,
pubmed-meshheading:10694496-RNA, Messenger,
pubmed-meshheading:10694496-Recombinant Proteins,
pubmed-meshheading:10694496-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10694496-Transforming Growth Factor beta,
pubmed-meshheading:10694496-Versicans
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Microvascular pericytes express aggrecan message which is regulated by BMP-2.
|
|
pubmed:affiliation |
McKay Laboratories for Orthopaedic Surgery Research, Department of Orthopaedic Surgery, School of Medicine, University of Pennsylvania, 36th and Hamilton Walk, Philadelphia, Pennsylvania 19104-6081, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
