Source:http://linkedlifedata.com/resource/pubmed/id/10679295
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2000-3-22
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| pubmed:abstractText |
Jagged-Notch interactions regulate a transmembrane ligand-receptor signaling pathway involved in the regulation of cell fate determination as well as myoblast and endothelial cell differentiation. To further examine the role of the transmembrane ligand, Jagged-1, in the regulation of cell differentiation, we stably transfected NIH 3T3 cells with a truncated form of Jagged(J)-1, which results in the secretion of a soluble(s) form of the protein. Comparison of gene expression by serial analysis demonstrated that among the 227 transcripts differentially regulated in the sJ-1 transfectants, the expression of the pro-alpha-2(I) collagen transcript and pro-alpha-1(I) collagen translation product was predominantly repressed in sJ-1 transfectants. When plated on extracellular matrices, sJ-1 transfectants formed prominent chord-like structures on type I collagen but not on fibrin, fibronectin, or vitronectin. While the sJ-1 transfectants exhibited growth kinetics similar to control cells and were unable to grow in soft agar, the cells were less sensitive to contact inhibition of growth in vitro and sJ-1 allografts formed tissue masses in nude mice after a prolonged latency period and exhibited an abundance of host-derived microvascular endothelial cells. These data suggest that J-1 may be able to modulate, in a matrix-dependent manner, the organization of cell to cell interactions including its ability to promote the development of chord-like structures.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serrate proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0006-291X
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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| pubmed:issnType |
Print
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| pubmed:day |
24
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| pubmed:volume |
268
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
853-9
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:10679295-3T3 Cells,
pubmed-meshheading:10679295-Animals,
pubmed-meshheading:10679295-Base Sequence,
pubmed-meshheading:10679295-Calcium-Binding Proteins,
pubmed-meshheading:10679295-Cell Communication,
pubmed-meshheading:10679295-Cell Differentiation,
pubmed-meshheading:10679295-Cell Division,
pubmed-meshheading:10679295-DNA Primers,
pubmed-meshheading:10679295-Extracellular Matrix,
pubmed-meshheading:10679295-Gene Expression,
pubmed-meshheading:10679295-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:10679295-Membrane Proteins,
pubmed-meshheading:10679295-Mice,
pubmed-meshheading:10679295-Mice, Nude,
pubmed-meshheading:10679295-Neovascularization, Physiologic,
pubmed-meshheading:10679295-Phenotype,
pubmed-meshheading:10679295-Proteins,
pubmed-meshheading:10679295-Signal Transduction,
pubmed-meshheading:10679295-Transfection
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| pubmed:year |
2000
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| pubmed:articleTitle |
A non-transmembrane form of Jagged-1 regulates the formation of matrix-dependent chord-like structures.
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| pubmed:affiliation |
Division of Hematology-Oncology, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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