Linked Life Data

1067896

Source:http://linkedlifedata.com/resource/pubmed/id/1067896

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11 Pt 1
pubmed:dateCreated
1976-12-30
pubmed:abstractText
The cloning efficiency of Friend erythroleukemic mouse cells is reduced by approximately 50% at every generation when the cells are continuously grown in the presence of 2% dimethyl sulfoxide (DMSO). Clones of murine erythroleukemic cells, resistant to the effect of the drug, have been selected from a nonmutagenized, hemoglobin (Hb)-inducible clone 745. The variant cells are present in the population with a frequency of 3 X 10(-5) cells. Twelve clones, characterized by different growth rates, have been examined in more detail. All the clones can grow in the continuous presence of 2% DMSO, are noninducible or poorly inducible by the drug, and consist of a homogeneous proerythroblastic cell population and a low number of Hb-synthesizing cells, except for clone R22S in which about 30% of the cells synthesize Hb. All but one (R2102) of the noninducible clones can be induced to synthesize Hb to various degrees by treatment with 1 mM butyric acid for 4 days. One clone (R1Z) forms colonies at a much higher efficiency in 2% DMSO than in nonselective plates, suggesting that it is conditional for DMSO. These and other resistant clones could be useful for the study of the mechanisms of induction of terminal differentiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4057-61
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
The phenotypes of variant clones of Friend mouse erythroleukemic cells resistant to dimethyl sulfoxide.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.