Linked Life Data

10671519

Source:http://linkedlifedata.com/resource/pubmed/id/10671519

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2000-3-21
pubmed:databankReference
pubmed:abstractText
The "MAN antigens" are polypeptides recognized by autoantibodies from a patient with a collagen vascular disease and localized to the nuclear envelope. We now show that one of the human MAN antigens termed MAN1 is a 82.3-kDa protein with an amino-terminal domain followed by two hydrophobic segments and a carboxyl-terminal tail. The MAN1 gene contains seven protein-coding exons and is assigned to human chromosome 12q14. Its mRNA is approximately 5.5 kilobases and is detected in several different cell types that were examined. Cell extraction experiments show that MAN1 is an integral membrane protein. When expressed in transfected cells, MAN1 is exclusively targeted to the nuclear envelope, consistent with an inner nuclear membrane localization. Protein sequence analysis reveals that MAN1 shares a conserved globular domain of approximately 40 amino acids, which we term the LEM module, with inner nuclear membrane proteins lamina-associated polypeptide 2 and emerin. The LEM module is also present in two proteins of Caenorhabditis elegans. These results show that MAN1 is an integral protein of the inner nuclear membrane that shares the LEM module with other proteins of this subcellular localization.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
275
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4840-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10671519-Amino Acid Sequence, pubmed-meshheading:10671519-Base Sequence, pubmed-meshheading:10671519-Caenorhabditis elegans Proteins, pubmed-meshheading:10671519-Chromosomes, Human, Pair 12, pubmed-meshheading:10671519-DNA, Complementary, pubmed-meshheading:10671519-DNA Primers, pubmed-meshheading:10671519-DNA-Binding Proteins, pubmed-meshheading:10671519-HeLa Cells, pubmed-meshheading:10671519-Humans, pubmed-meshheading:10671519-Membrane Proteins, pubmed-meshheading:10671519-Microscopy, Fluorescence, pubmed-meshheading:10671519-Molecular Sequence Data, pubmed-meshheading:10671519-Nuclear Envelope, pubmed-meshheading:10671519-Nuclear Proteins, pubmed-meshheading:10671519-RNA, Messenger, pubmed-meshheading:10671519-Sequence Homology, Amino Acid, pubmed-meshheading:10671519-Thymopoietins
pubmed:year
2000
pubmed:articleTitle
MAN1, an inner nuclear membrane protein that shares the LEM domain with lamina-associated polypeptide 2 and emerin.
pubmed:affiliation
Departments of Medicine and of Anatomy and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't