10666409

Source:http://linkedlifedata.com/resource/pubmed/id/10666409

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0018787, umls-concept:C0034493, umls-concept:C0205409, umls-concept:C0332120, umls-concept:C0376466, umls-concept:C1120843, umls-concept:C1314939, umls-concept:C1442514, umls-concept:C1515877, umls-concept:C1879547
pubmed:issue	2
pubmed:dateCreated	2000-2-24
pubmed:abstractText	Recent studies suggest that p38 mitogen-activated protein kinase (MAPK) may be involved in ischemic preconditioning (PC). To further test this possibility, the regulation of MAPK-activated protein kinase 2 (MAPKAPK2), a kinase immediately downstream from p38 MAPK, and the activity of c-Jun NH(2)-terminal kinase (JNK), a second MAPK, were examined in preconditioned hearts. Isolated, perfused rabbit hearts were subjected to 20 to 30 minutes of global ischemia. Ventricular biopsies before treatment and after 20 minutes of ischemia were homogenized, and the activities of MAPKAPK2 and JNK were evaluated. For the MAPKAPK2 experiments, 7 groups were studied, as follows: control hearts; preconditioned hearts; hearts treated with 500 nmol/L R(-) N(6)-(2-phenylisopropyl) adenosine (PIA), an A(1)-adenosine receptor agonist; preconditioned hearts pretreated with 100 micromol/L 8-(p-sulfophenyl) theophylline (SPT), an adenosine receptor antagonist; preconditioned hearts also treated with SB 203580, a potent inhibitor of p38 MAPK activation; hearts treated with 50 ng/mL anisomycin (a p38 MAPK/JNK activator); and hearts treated with both anisomycin (50 ng/mL) and the tyrosine kinase inhibitor genistein (50 micromol/L). MAPKAPK2 activity was not altered in control hearts after 20 minutes of global ischemia. By contrast, there was a 3.8-fold increase in activity during ischemia in preconditioned hearts. Activation of MAPKAPK2 in preconditioned hearts was blocked by both SPT and SB 203580. MAPKAPK2 activity during ischemia increased 3.5-fold and 3.3-fold in hearts pretreated with PIA or anisomycin, respectively. MAPKAPK2 activation during ischemia in hearts pretreated with anisomycin was blocked by genistein. In separate hearts, anisomycin mimicked the anti-infarct effect of PC, and that protection was abolished by genistein. JNK activity was measured in control and preconditioned hearts. There was a comparable, modest decline in activity during 30 minutes of global ischemia in both groups. As a positive control, a third group of hearts was treated with anisomycin before global ischemia, and in these, JNK activity increased by 290% above baseline. These results confirm that the p38 MAPK/MAPKAPK2 pathway is activated during ischemia only if the heart is in a preconditioned state. These data further support p38 MAPK as an important signaling component in ischemic PC.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/0047103
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Anisomycin, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Genistein, http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides..., http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein..., http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase 4, http://linkedlifedata.com/resource/pubmed/chemical/MAP-kinase-activated kinase 2, http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase..., http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Protein Synthesis Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
pubmed:status	MEDLINE
pubmed:month	Feb
pubmed:issn	1524-4571
pubmed:author	pubmed-author:BainesC PCP, pubmed-author:CHUJ SJS, pubmed-author:CohenM VMV, pubmed-author:CritzS DSD, pubmed-author:DownerJ DJD, pubmed-author:NakanoAA, pubmed-author:PelechS LSL
pubmed:issnType	Electronic
pubmed:day	4
pubmed:volume	86
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	144-51
pubmed:dateRevised	2009-11-19
pubmed:meshHeading	pubmed-meshheading:10666409-Animals, pubmed-meshheading:10666409-Anisomycin, pubmed-meshheading:10666409-Coronary Circulation, pubmed-meshheading:10666409-Enzyme Activation, pubmed-meshheading:10666409-Enzyme Inhibitors, pubmed-meshheading:10666409-Genistein, pubmed-meshheading:10666409-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:10666409-Ischemic Preconditioning, Myocardial, pubmed-meshheading:10666409-JNK Mitogen-Activated Protein Kinases, pubmed-meshheading:10666409-MAP Kinase Kinase 4, pubmed-meshheading:10666409-MAP Kinase Signaling System, pubmed-meshheading:10666409-Mitogen-Activated Protein Kinase Kinases, pubmed-meshheading:10666409-Mitogen-Activated Protein Kinases, pubmed-meshheading:10666409-Myocardial Infarction, pubmed-meshheading:10666409-Myocardial Ischemia, pubmed-meshheading:10666409-Myocardium, pubmed-meshheading:10666409-Protein Synthesis Inhibitors, pubmed-meshheading:10666409-Protein-Serine-Threonine Kinases, pubmed-meshheading:10666409-Rabbits, pubmed-meshheading:10666409-p38 Mitogen-Activated Protein Kinases
pubmed:year	2000
pubmed:articleTitle	Ischemic preconditioning activates MAPKAPK2 in the isolated rabbit heart: evidence for involvement of p38 MAPK.
pubmed:affiliation	Department of Physiology, University of South Alabama, Mobile, AL 36688-0002, USA.
pubmed:publicationType	Journal Article, In Vitro