Source:http://linkedlifedata.com/resource/pubmed/id/10665800
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2000-2-17
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| pubmed:abstractText |
The venom of the green mamba, Dendroaspis angusticeps, was found to have at least six trace m1-specific toxins that block the binding of 3H-N-methylscopolamine to cloned m1 muscarinic receptors. Four were isolated by gel filtration, cation exchange HPLC and reversed-phase HPLC and named m1-toxins1-4. Recovery was 180, 90, 20 and 10 microg/g dry venom, respectively. m1-Toxin1 (the original m1-toxin) was found to have the sequence, L T C V K S N S I W F P T S E D C P D G Q N LC F K R W Q Y I S P R M Y D F T R G C A A T C P K A E Y R D V I N C C G T D K C N K, calculated mass = 7473 Da and calculated pI = 8.2. This sequence had been predicted previously from a cDNA cloned from the venom glands of this snake. The binding of m1-toxin1 was irreversible, so its Kd could not be determined. m1-Toxin2 differed only in proline-19, mass = 7455 and pI = 8.5. Partial sequence data for m1-toxin3 showed aspartate-7 and m1-toxin4 showed isoleucine-12, asparagine-16 and alanine-19. m1-Toxins1-4 have seven conserved amino acids not found in homologous mamba toxins that bind to other muscarinic receptors (MT1, MT2, m4-toxin = MT3, MT4, MT5, MTalpha and MTbeta). Some of these residues may be essential for m1-specificity. Since m1-toxin1 binds irreversibly in artificial cerebrospinal fluid at 37 degrees C, it is a particularly attractive antagonist for studies in vivo.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0041-0101
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
38
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
187-98
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| pubmed:dateRevised |
2005-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10665800-Amino Acid Sequence,
pubmed-meshheading:10665800-Animals,
pubmed-meshheading:10665800-CHO Cells,
pubmed-meshheading:10665800-Chromatography, High Pressure Liquid,
pubmed-meshheading:10665800-Cricetinae,
pubmed-meshheading:10665800-Elapid Venoms,
pubmed-meshheading:10665800-Elapidae,
pubmed-meshheading:10665800-Molecular Sequence Data,
pubmed-meshheading:10665800-Muscarinic Antagonists,
pubmed-meshheading:10665800-Protein Folding,
pubmed-meshheading:10665800-Receptor, Muscarinic M1,
pubmed-meshheading:10665800-Receptors, Muscarinic
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
m1-toxin isotoxins from the green mamba (Dendroaspis angusticeps) that selectively block m1 muscarinic receptors.
|
| pubmed:affiliation |
Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, FL 33101, USA.
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| pubmed:publicationType |
Journal Article
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