Linked Life Data

10657706

Source:http://linkedlifedata.com/resource/pubmed/id/10657706

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2000-4-11
pubmed:abstractText
In situ detection of neural progenitor cells including stem-like cells is essential for studying the basic mechanisms of the generation of cellular diversity in the CNS, upon which therapeutic treatments for CNS injuries, degenerative diseases, and brain tumors may be based. We have generated rat monoclonal antibodies (Mab 14H1 and 14B8) that recognize an RNA-binding protein Musashi1, but not a Musashi1-related protein, Musashi2. The amino acid sequences at the epitope sites of these anti-Musashi1 Mabs are remarkably conserved among the human, mouse, and Xenopus proteins. Spatiotemporal patterns of Musashi1 immunoreactivity in the developing and/or adult CNS tissues of frogs, birds, rodents, and humans indicated that our anti-Musashi1 Mabs reacted with undifferentiated, proliferative cells in the CNS of all the vertebrates tested. Double or triple immunostaining of embryonic mouse brain cells in monolayer cultures demonstrated strong Musashi1 expression in Nestin(+)/RC2(+) cells. The relative number of Musashi1(+)/Nestin(+)/RC2(+) cells increased fivefold when embryonic forebrain cells were cultured to form 'neurospheres' in which stem-like cells are known to be enriched through their self-renewing mode of growth. Nestin(+)/RC2(-) cells, which included Talpha1-GFP(+) neuronal progenitor cells and GLAST(+) astroglial precursor cells, were also Musashi1(+), as were GFAP(+) astrocytes. Young neurons showed a trace of Musashi1 expression. Cells committed to the oligodendroglial lineage were Musashi(-). Musashi1 was localized to the perikarya of CNS stem-like cells and non-oligodendroglial progenitor cells without shifting to cell processes or endfeet, and is therefore advantageous for identifying each cell and counting cells in situ.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0378-5866
pubmed:author
pubmed:copyrightInfo
Copyright 2000 S. Karger AG, Basel.
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
139-53
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:10657706-Amino Acid Sequence, pubmed-meshheading:10657706-Animals, pubmed-meshheading:10657706-Antibodies, Monoclonal, pubmed-meshheading:10657706-Biological Evolution, pubmed-meshheading:10657706-Central Nervous System, pubmed-meshheading:10657706-Chick Embryo, pubmed-meshheading:10657706-Conserved Sequence, pubmed-meshheading:10657706-Cytological Techniques, pubmed-meshheading:10657706-Epitopes, pubmed-meshheading:10657706-Genetic Markers, pubmed-meshheading:10657706-Mice, pubmed-meshheading:10657706-Molecular Sequence Data, pubmed-meshheading:10657706-Nerve Tissue Proteins, pubmed-meshheading:10657706-Neurons, pubmed-meshheading:10657706-RNA-Binding Proteins, pubmed-meshheading:10657706-Stem Cells, pubmed-meshheading:10657706-Tissue Distribution, pubmed-meshheading:10657706-Xenopus, pubmed-meshheading:10657706-Xenopus Proteins
pubmed:year
2000
pubmed:articleTitle
Musashi1: an evolutionally conserved marker for CNS progenitor cells including neural stem cells.
pubmed:affiliation
Division of Neuroanatomy, Department of Neuroscience, Biomedical Research Center, Osaka University Graduate School of Medicine, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't