Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-3-16
pubmed:databankReference
pubmed:abstractText
The rapid identification of bacteria in blood cultures and other clinical specimens is important for patient management and antimicrobial therapy. We describe a rapid (<4 h) detection and identification system that uses universal PCR primers to amplify a variable region of bacterial 23S ribosomal DNA, followed by reverse hybridization of the products to a panel of oligonucleotides. This procedure was successful in discriminating a range of bacteria in pure cultures. When this procedure was applied directly to 158 unselected positive blood culture broths on the day when growth was detected, 125 (79.7%) were correctly identified, including 4 with mixed cultures. Nine (7.2%) yielded bacteria for which no oligonucleotide targets were present in the oligonucleotide panel, and 16 culture-positive broths (10.3%) produced no PCR product. In seven of the remaining eight broths, streptococci were identified but not subsequently grown, and one isolate of Staphylococcus aureus was misidentified as a coagulase-negative staphylococcus. The accuracy, range, and discriminatory power of the assay can be continually extended by adding further oligonucleotides to the panel without significantly increasing complexity or cost.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-1326592, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-1580579, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-1939570, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-1969433, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-2061297, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-2189856, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-2406020, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-2439888, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-2583187, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-2745686, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-2762325, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-7512093, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-7598144, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-7639408, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-7844395, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-8197176, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-8207173, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-8314983, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-8581168, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-8681987, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-8879774, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-8904404, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-8904424, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-8950560, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9011049, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9041409, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9076711, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9163461, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9254694, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9361884, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9431924, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9445103, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9500808, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9508293, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9516883, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9565407, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9571742, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9738025, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9758055, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655385-9845853
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
781-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Rapid diagnosis of bacteremia by universal amplification of 23S ribosomal DNA followed by hybridization to an oligonucleotide array.
pubmed:affiliation
Department of Microbiology, King's College St. Thomas' Campus, St. Thomas' Hospital, London SE1 7EH, United Kingdom.
pubmed:publicationType
Journal Article