rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
12
|
pubmed:dateCreated |
2000-2-11
|
pubmed:abstractText |
One striking disadvantage of in vitro culturing of human retinal pigment epithelial (RPE) cells is the loss of epithelial differentiation and specific cell function during culture. This may be one of the main reasons for the failure of RPE cell transplantation. The aim of this study was to evaluate cell culture conditions ensuring the maintenance of differentiation and function of RPE cells after subcultivation and storage in liquid nitrogen.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0721-832X
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
237
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1001-6
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:10654169-Adolescent,
pubmed-meshheading:10654169-Adult,
pubmed-meshheading:10654169-Aged,
pubmed-meshheading:10654169-Calcium Channels,
pubmed-meshheading:10654169-Cell Transplantation,
pubmed-meshheading:10654169-Cells, Cultured,
pubmed-meshheading:10654169-Child,
pubmed-meshheading:10654169-Cryopreservation,
pubmed-meshheading:10654169-Culture Media, Conditioned,
pubmed-meshheading:10654169-HLA Antigens,
pubmed-meshheading:10654169-Histocompatibility Testing,
pubmed-meshheading:10654169-Humans,
pubmed-meshheading:10654169-Middle Aged,
pubmed-meshheading:10654169-Organ Preservation,
pubmed-meshheading:10654169-Patch-Clamp Techniques,
pubmed-meshheading:10654169-Pigment Epithelium of Eye,
pubmed-meshheading:10654169-Tissue Donors
|
pubmed:year |
1999
|
pubmed:articleTitle |
Physiological features of primary cultures and subcultures of human retinal pigment epithelial cells before and after cryopreservation for cell transplantation.
|
pubmed:affiliation |
Department of Ophthalmology, University of Hamburg, Germany.
|
pubmed:publicationType |
Journal Article,
Comparative Study
|