Linked Life Data

10650976

Source:http://linkedlifedata.com/resource/pubmed/id/10650976

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-2-28
pubmed:abstractText
In the hippocampus, pyramidal cells are more vulnerable than granule cells and interneurones to energy depletion during hypoxia and ischaemia. The aim of the present study was to explore whether this difference is related to the lower expression of adenosine 5'-triphosphate-sensitive potassium (K(ATP)) channels in pyramidal cells compared to other hippocampal neurones. Hippocampal slices were prepared from 10- to 13-day-old rats, and CAI pyramidal cells and interneurones of the stratum radiatum were visually and electrophysiologically identified. Energy depletion was produced by removing glucose from the bath or by inhibiting mitochondrial metabolism using rotenone. In the perforated-patch configuration, both protocols elicited outward currents in only a minority of the pyramidal cells but in most of the interneurones. The currents started to develop 9-57 min after glucose deprivation and 4-16 min after rotenone application and reversed near the K+ equilibrium potential. Bath-applied diazoxide (0.3 mM), an opener of K(ATP) channels, could activate additional currents. The sulphonylureas tolbutamide (0.5 mM) or glibenclamide (20 microM), two blockers of K(ATP) channels, totally inhibited the currents induced by energy depletion and activated by diazoxide. The results demonstrate the differential activation of K(ATP) channels during energy depletion in pyramidal cells and interneurones, and suggest that channel activation is neuroprotective against the deleterious effects of energy depletion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
439
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
256-62
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:10650976-ATP-Binding Cassette Transporters, pubmed-meshheading:10650976-Animals, pubmed-meshheading:10650976-Diazoxide, pubmed-meshheading:10650976-Electric Stimulation, pubmed-meshheading:10650976-Electrophysiology, pubmed-meshheading:10650976-Energy Metabolism, pubmed-meshheading:10650976-Glucose, pubmed-meshheading:10650976-Hippocampus, pubmed-meshheading:10650976-Interneurons, pubmed-meshheading:10650976-KATP Channels, pubmed-meshheading:10650976-Patch-Clamp Techniques, pubmed-meshheading:10650976-Potassium Channel Blockers, pubmed-meshheading:10650976-Potassium Channels, pubmed-meshheading:10650976-Potassium Channels, Inwardly Rectifying, pubmed-meshheading:10650976-Pyramidal Cells, pubmed-meshheading:10650976-Rats, pubmed-meshheading:10650976-Rats, Wistar, pubmed-meshheading:10650976-Rotenone, pubmed-meshheading:10650976-Sulfonylurea Compounds, pubmed-meshheading:10650976-Uncoupling Agents
pubmed:year
2000
pubmed:articleTitle
Differential activation of ATP-sensitive potassium channels during energy depletion in CA1 pyramidal cells and interneurones of rat hippocampus.
pubmed:affiliation
I. Physiologisches Institut, Universität des Saarlandes, Homburg, Germany. phczaw@med-rz.uni-sb.de
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't