Linked Life Data

10650126

Source:http://linkedlifedata.com/resource/pubmed/id/10650126

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-3-17
pubmed:abstractText
The Golgi apparatus (GA) of innervated rat and chicken skeletal muscle is present in a typical perinuclear location, and in subsynaptic areas where it disperses after denervation. It was suggested that the subsynaptic segments of the GA are linked with functions involved in the maturation and targeting of synaptic proteins. Similarly, the GA of rat myocardium is found in a perinuclear location and between myofibrils, adjacent to the T system of tubules. These findings raise the question whether the GA of polarized cells is present in a typical perinuclear location, for the performance of general "housekeeping" functions, and in distal areas, for the mediation of specialized functions. Astrocytes may contain GA within their long cytoplasmic processes which are difficult to identify in thin sections. To ensure the astrocytic origin of GA in otherwise unidentifiable small processes, we used transgenic mice expressing the rat MG160 medial Golgi sialoglycoprotein only in the GA of astrocytes, and visualized the GA with monoclonal antibody 10A8 (mAb10A8) which reacts only with rat MG160. Thus, we identified cisternae of the GA in distal perisynaptic and subependymal processes, in perivascular foot plates of cerebral astrocytes, and in processes of the Müller glia in the retina. A similar strategy may be adopted in future investigations aiming at the detection of elements of the GA in distal processes of neurons and oligodendrocytes. The functional implications of GA in perisynaptic astrocytic processes and other processes are unknown. However, the isolation and molecular characterization of the perisynaptic subset of astrocytic Golgi may be feasible, since others have purified the astrocytic glutamate transporter 1 (GLT1) from crude synaptosomal fractions in which astrocytic processes are probably unavoidable contaminants.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
855
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
23-31
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10650126-Animals, pubmed-meshheading:10650126-Antibodies, Monoclonal, pubmed-meshheading:10650126-Antibody Specificity, pubmed-meshheading:10650126-Astrocytes, pubmed-meshheading:10650126-Ependyma, pubmed-meshheading:10650126-Golgi Apparatus, pubmed-meshheading:10650126-Membrane Proteins, pubmed-meshheading:10650126-Mice, pubmed-meshheading:10650126-Mice, Transgenic, pubmed-meshheading:10650126-Microglia, pubmed-meshheading:10650126-Microscopy, Electron, pubmed-meshheading:10650126-Neuropil, pubmed-meshheading:10650126-Oligodendroglia, pubmed-meshheading:10650126-Rats, pubmed-meshheading:10650126-Receptors, Cell Surface, pubmed-meshheading:10650126-Receptors, Fibroblast Growth Factor, pubmed-meshheading:10650126-Retina, pubmed-meshheading:10650126-Sialoglycoproteins, pubmed-meshheading:10650126-Synapses
pubmed:year
2000
pubmed:articleTitle
The golgi apparatus is present in perisynaptic, subependymal and perivascular processes of astrocytes and in processes of retinal Müller glia.
pubmed:affiliation
Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, 418 Johnson Pavilion, 36 Hamilton Walk, Philadelphia, PA 19104-6079, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.