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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1976-9-2
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pubmed:abstractText |
The trace element selenium is essential for clonal growth of diploid fibroblasts from human fetal lung (WI-38) in media containing small amounts of serum protein. Maximum growth stimulation is obtained when 30 nM neutralized selenious acid is added to a synthetic medium containing 1.5 mg/ml of dialyzed fetal bovine serum protein (equivalent to a 3% serum concentration). Serum appears to be a source of selenium in most culture media, since higher concentrations of serum protein or whole serum mask the selenium requirement of WI-38 cells. Selenium is also required by a Chinese hamster cell line that can be grown in a protein-free synthetic culture medium.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-13286432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-13592294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-13727165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-13952249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-13952250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-14294058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-14315085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-17421739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-4120885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-4206269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-4208659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-4585079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-4605100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-4617404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-4731946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-4736708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-4840827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-803996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064872-807152
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
73
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2023-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1976
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pubmed:articleTitle |
Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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