Linked Life Data

10648518

Source:http://linkedlifedata.com/resource/pubmed/id/10648518

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-2-23
pubmed:databankReference
pubmed:abstractText
A 4.2-kb PstI fragment harboring the gene cluster of the ribulose monophosphate (RuMP) pathway for formaldehyde fixation was identified in the chromosome of a gram-positive, facultative methylotroph, Mycobacterium gastri MB19, by using the coding region of 3-hexulose-6-phosphate synthase (HPS) as the hybridization probe. The PstI fragment contained three complete open reading frames (ORFs) which encoded from the 5' end, a DNA-binding regulatory protein (rmpR), 6-phospho-3-hexuloisomerase (PHI; rmpB), and HPS (rmpA). Sequence analysis suggested that rmpA and rmpB constitute an operon, and Northern blot analysis of RNA extracted from bacteria grown under various conditions suggested that the expression of the two genes is similarly regulated at the transcriptional level. A similarity search revealed that the proteins encoded by rmpA and rmpB in M. gastri MB19 show high similarity to the unidentified proteins of nonmethylotrophic prokaryotes, including bacteria and anaerobic archaea. The clusters in the phylogenetic tree of the HPS protein of M. gastri MB19 and those in the phylogenetic tree of the PHI protein were nearly identical, which implies that these two formaldehyde-fixing genes evolved as a pair. These findings give new insight into the acquisition of the formaldehyde fixation pathway during the evolution of diverse microorganisms.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-10361681, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-10572115, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-1510560, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-2060763, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-2280712, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-353476, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-7556189, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-7966270, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-7984417, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-8566707, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-8595859, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-9150882, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-9384377, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-9409145, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-9537375, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-9651254, http://linkedlifedata.com/resource/pubmed/commentcorrection/10648518-9811645
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
182
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
944-8
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
A novel operon encoding formaldehyde fixation: the ribulose monophosphate pathway in the gram-positive facultative methylotrophic bacterium Mycobacterium gastri MB19.
pubmed:affiliation
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Sakyo-ku, Kyoto 606-8502, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't