Linked Life Data

10646947

Source:http://linkedlifedata.com/resource/pubmed/id/10646947

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-2-2
pubmed:abstractText
The dissolution behavior of hydroxyapatite (HA) in the presence and absence of protein needs to be investigated in order to fully understand the initial cellular response to HA surfaces. In this study, HA coatings were characterized using X-ray diffraction, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy (FTIR) prior to protein study. Fibronectin and albumin adsorption study were also performed. Calcium and phosphorus released in the presence and absence of albumin were measured. pH of the solution was measured daily. From the materials characterization, it was observed that the coatings exhibit a HA-type structure, with traces of sodium on the surface. FTIR indicated the possible presence of carbonates on the coatings. From the adsorption study, the amount of albumin adsorbed (0.052+/-0.005 microg/mm2) was statistically higher than the amount of fibronectin adsorbed on HA surfaces (0.035+/-0.002 microg/mm2). Flame atomic absorption indicated a significantly higher calcium ions released initially for HA coatings incubated with proteins as compared to coatings in the absence of proteins. However, after 7 days incubation, no significant difference in calcium ions release was observed between the HA coatings in the presence and absence of proteins. Phosphorus dissolution on HA coatings was not significantly affected by the presence of proteins. Thus, it was suggested from this study that the initial dissolution properties of calcium ions from HA coatings was dependent on the media.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0142-9612
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
299-305
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Effect of protein on the dissolution of HA coatings.
pubmed:affiliation
Department of Restorative Dentistry, University of Texas Health Science Center at San Antonio, 78284-7890, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't