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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-3-2
pubmed:abstractText
Proinflammatory cytokines, including IL-1beta and tumor necrosis factor-alpha (TNF-alpha), promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1 (VCAM-1) on hepatic sinusoidal endothelium (HSE). In this study, hepatic metastasis after intrasplenically injected mouse B16 melanoma (B16M) cells was reduced 84-95% in mice with null mutations for either IL-1beta or the IL-1beta-converting enzyme (ICE, caspase-1) compared with wild-type mice. On day 12, 47% of wild-type mice were dead compared with 19% of either IL-1beta or ICE-deficient mice. In vitro, conditioned medium from B16M cells (B16M-CM) induced the release of TNF-alpha and IL-1beta from cultures of primary murine HSE. The effect of B16M-CM on HSE resulted in increased numbers of B16M cells adhering to HSE, which was completely abrogated by a specific inhibitor of ICE, anti-IL-18 or IL-18-binding protein. Exogenous IL-18 added to HSE also increased the number of adhering melanoma cells; however, this was not affected by IL-1 receptor blockade or TNF neutralization but rather by anti-VCAM-1. These results demonstrate a role for IL-1beta and IL-18 in the development of hepatic metastases of B16M in vivo. In vitro, soluble products from B16M cells stimulate HSE to sequentially release TNF-alpha, IL-1beta, and IL-18. The IL-18 cytokine increases expression of VCAM-1 and the adherence of melanoma cells.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-10023777, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-10051628, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-10070959, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-1143319, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-1680022, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-1988686, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-2148875, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-2153136, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-2293911, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-2492265, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-2588007, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-7477296, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-7515767, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-7529137, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-7535475, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-7621081, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-7682484, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-7774220, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-7896459, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-8168095, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-8267795, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-8340061, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-8478614, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-8632494, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-8999548, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-9029121, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-9096586, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-9121587, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-9261550, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-9449707, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-9462694, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-9469432, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-9490698, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-9502787, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-9529155, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-9565638, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-9766633, http://linkedlifedata.com/resource/pubmed/commentcorrection/10639148-9893178
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
734-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
IL-18 regulates IL-1beta-dependent hepatic melanoma metastasis via vascular cell adhesion molecule-1.
pubmed:affiliation
School of Medicine and Dentistry, University of the Basque Country, 48940 Vizcaya, Spain.
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