Linked Life Data

10635600

Source:http://linkedlifedata.com/resource/pubmed/id/10635600

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2000-2-4
pubmed:abstractText
The methods normally used for the detection of enteroviruses in environmental samples involve the use of cell cultures, which are expensive and time consuming. The Polymerase Chain Reaction (PCR) is a useful tool for the detection of enteroviruses in several matrixes because primary cell culture is not needed and the increased sensitivity of PCR allows detection of the low numbers of target nucleic acids usually found in environmental samples. A 5-h, user-friendly PCR assay was used to detect enteroviruses in bivalves molluscs (clams) and sewage. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylase. Detection was performed colorimetrically in a microwell titer plate. This method has greater advantages over conventional methodologies for routinely screening a large number of samples, namely, the rapid acquisition of results and cost effectiveness.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0048-9697
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
243-244
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
285-9
pubmed:dateRevised
2006-4-19
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Simple method of detecting enteroviruses in contaminated molluscs and sewage by using polymerase chain reaction coupled with a colorimetric microwell detection assay.
pubmed:affiliation
Dipartimento di Farmacologia Sperimentale, Università degli Studi di Napoli Federico II, Italy. gualillo@usc.es
pubmed:publicationType
Journal Article