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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5451
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pubmed:dateCreated |
2000-2-1
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pubmed:abstractText |
Abscisic acid (ABA) stimulates stomatal closure and thus supports water conservation by plants during drought. Mass spectrometry-generated peptide sequence information was used to clone a Vicia faba complementary DNA, AAPK, encoding a guard cell-specific ABA-activated serine-threonine protein kinase (AAPK). Expression in transformed guard cells of AAPK altered by one amino acid (lysine 43 to alanine 43) renders stomata insensitive to ABA-induced closure by eliminating ABA activation of plasma membrane anion channels. This information should allow cell-specific, targeted biotechnological manipulation of crop water status.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
287
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
300-3
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pubmed:dateRevised |
2010-2-2
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pubmed:meshHeading |
pubmed-meshheading:10634783-Abscisic Acid,
pubmed-meshheading:10634783-Amino Acid Sequence,
pubmed-meshheading:10634783-Anions,
pubmed-meshheading:10634783-Biolistics,
pubmed-meshheading:10634783-Cloning, Molecular,
pubmed-meshheading:10634783-DNA, Complementary,
pubmed-meshheading:10634783-Enzyme Activation,
pubmed-meshheading:10634783-Fabaceae,
pubmed-meshheading:10634783-Genes, Plant,
pubmed-meshheading:10634783-Ion Channels,
pubmed-meshheading:10634783-Molecular Sequence Data,
pubmed-meshheading:10634783-Mutagenesis, Site-Directed,
pubmed-meshheading:10634783-Patch-Clamp Techniques,
pubmed-meshheading:10634783-Plant Leaves,
pubmed-meshheading:10634783-Plant Proteins,
pubmed-meshheading:10634783-Plants, Medicinal,
pubmed-meshheading:10634783-Protein-Serine-Threonine Kinases,
pubmed-meshheading:10634783-Protoplasts,
pubmed-meshheading:10634783-Recombinant Fusion Proteins,
pubmed-meshheading:10634783-Transformation, Genetic
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pubmed:year |
2000
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pubmed:articleTitle |
Regulation of abscisic acid-induced stomatal closure and anion channels by guard cell AAPK kinase.
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pubmed:affiliation |
Department of Biology, The Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16802, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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