| pubmed-article:10626681 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10626681 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:10626681 | lifeskim:mentions | umls-concept:C0665952 | lld:lifeskim |
| pubmed-article:10626681 | lifeskim:mentions | umls-concept:C1416645 | lld:lifeskim |
| pubmed-article:10626681 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:10626681 | pubmed:dateCreated | 2000-2-11 | lld:pubmed |
| pubmed-article:10626681 | pubmed:abstractText | Human natural killer (NK) cells have receptors that bind to HLA class I molecules. Among these receptors, p58 and p50 bind to HLA-C. P58 belongs to the killer inhibitory receptor (KIR) and p50 belongs to the killer activatory receptor (KAR). Previously, we obtained three recombinant p58 KIR or p50 KAR proteins, KAR-K1 (KIR2DS4), KIR-K6 (KIR2DL1), and KIR-K7 (KIR2DL3). In this study, we produced and characterized seven monoclonal antibodies (MAbs) to the p58 KIR and p50 KAR proteins. The MAbs were classified in three groups according to their antigen-binding specificity: 5IB103 and 26ID707 were KAR-K1-specific; A809, 190IIC311, and 197IIC611 were KIR-K7-specific; while A210 and A803g bound to all three recombinant proteins. The MAbs reactive to KIR-K7 bound to the gamma3 domain among two immunoglobulin (Ig) domains of KIR-K7. Immunofluorescence staining and flow cytometric analysis with A803g showed reactivity to about 10% of peripheral blood mononuclear cells and 35% of purified natural killer cells. Double immunofluorescence staining with A803g and anti-CD56 Ab showed that CD56 and p58 or p50 were expressed on NK cells in a mutually exclusive way. We also investigated T-cell markers in A803g+ cells. A803g+ T cells were almost CD8+ cells. MAbs produced in this study can be utilized practically in the investigation of biological characteristics of p58 KIR and p50 KAR. | lld:pubmed |
| pubmed-article:10626681 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10626681 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10626681 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10626681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10626681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10626681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10626681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10626681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10626681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10626681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10626681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10626681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10626681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10626681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10626681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10626681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10626681 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10626681 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10626681 | pubmed:issn | 0272-457X | lld:pubmed |
| pubmed-article:10626681 | pubmed:author | pubmed-author:KitSS | lld:pubmed |
| pubmed-article:10626681 | pubmed:author | pubmed-author:KimS JSJ | lld:pubmed |
| pubmed-article:10626681 | pubmed:author | pubmed-author:ParkJ HJH | lld:pubmed |
| pubmed-article:10626681 | pubmed:author | pubmed-author:ChoiI HIH | lld:pubmed |
| pubmed-article:10626681 | pubmed:author | pubmed-author:ShinJ SJS | lld:pubmed |
| pubmed-article:10626681 | pubmed:author | pubmed-author:ShinE CEC | lld:pubmed |
| pubmed-article:10626681 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10626681 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:10626681 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10626681 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10626681 | pubmed:pagination | 521-7 | lld:pubmed |
| pubmed-article:10626681 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:10626681 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10626681 | pubmed:articleTitle | Monoclonal antibodies with various reactivity to p58 killer inhibitory receptors. | lld:pubmed |
| pubmed-article:10626681 | pubmed:affiliation | Department of Microbiology, Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul, Korea. | lld:pubmed |
| pubmed-article:10626681 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10626681 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
