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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2000-2-11
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pubmed:abstractText |
Human natural killer (NK) cells have receptors that bind to HLA class I molecules. Among these receptors, p58 and p50 bind to HLA-C. P58 belongs to the killer inhibitory receptor (KIR) and p50 belongs to the killer activatory receptor (KAR). Previously, we obtained three recombinant p58 KIR or p50 KAR proteins, KAR-K1 (KIR2DS4), KIR-K6 (KIR2DL1), and KIR-K7 (KIR2DL3). In this study, we produced and characterized seven monoclonal antibodies (MAbs) to the p58 KIR and p50 KAR proteins. The MAbs were classified in three groups according to their antigen-binding specificity: 5IB103 and 26ID707 were KAR-K1-specific; A809, 190IIC311, and 197IIC611 were KIR-K7-specific; while A210 and A803g bound to all three recombinant proteins. The MAbs reactive to KIR-K7 bound to the gamma3 domain among two immunoglobulin (Ig) domains of KIR-K7. Immunofluorescence staining and flow cytometric analysis with A803g showed reactivity to about 10% of peripheral blood mononuclear cells and 35% of purified natural killer cells. Double immunofluorescence staining with A803g and anti-CD56 Ab showed that CD56 and p58 or p50 were expressed on NK cells in a mutually exclusive way. We also investigated T-cell markers in A803g+ cells. A803g+ T cells were almost CD8+ cells. MAbs produced in this study can be utilized practically in the investigation of biological characteristics of p58 KIR and p50 KAR.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD56,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/KIR2DL3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgG,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, KIR,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, KIR2DL1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, KIR2DL3,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Natural Killer Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0272-457X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
521-7
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10626681-Animals,
pubmed-meshheading:10626681-Antibodies, Monoclonal,
pubmed-meshheading:10626681-Antibody Specificity,
pubmed-meshheading:10626681-Antigens, CD4,
pubmed-meshheading:10626681-Antigens, CD56,
pubmed-meshheading:10626681-Antigens, CD8,
pubmed-meshheading:10626681-Blotting, Western,
pubmed-meshheading:10626681-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:10626681-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10626681-Epitopes,
pubmed-meshheading:10626681-Hybridomas,
pubmed-meshheading:10626681-Killer Cells, Natural,
pubmed-meshheading:10626681-Leukocytes, Mononuclear,
pubmed-meshheading:10626681-Mice,
pubmed-meshheading:10626681-Mice, Inbred BALB C,
pubmed-meshheading:10626681-Receptors, IgG,
pubmed-meshheading:10626681-Receptors, Immunologic,
pubmed-meshheading:10626681-Receptors, KIR,
pubmed-meshheading:10626681-Receptors, KIR2DL1,
pubmed-meshheading:10626681-Receptors, KIR2DL3,
pubmed-meshheading:10626681-Receptors, Natural Killer Cell,
pubmed-meshheading:10626681-Recombinant Proteins,
pubmed-meshheading:10626681-T-Lymphocyte Subsets
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pubmed:year |
1999
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pubmed:articleTitle |
Monoclonal antibodies with various reactivity to p58 killer inhibitory receptors.
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pubmed:affiliation |
Department of Microbiology, Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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