Linked Life Data

10616716

Source:http://linkedlifedata.com/resource/pubmed/id/10616716

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pubmed-article:10616716pubmed:abstractTextAutoselective xylose-utilising strains of Saccharomyces cerevisiae expressing the xylose reductase (XYL1) and xylitol dehydrogenase (XYL2) genes of Pichia stipitis were constructed by replacing the chromosomal FUR1 gene with a disrupted fur1::LEU2 allele. Anaerobic fermentations with 80 g l-1 D-xylose as substrate showed a twofold higher consumption of xylose in complex medium compared to defined medium. The xylose consumption rate increased a further threefold when 20 g l-1 D-glucose or raffinose was used as co-substrate together with 50 g l-1 D-xylose. Xylose consumption was higher with raffinose as co-substrate than with glucose (85% versus 71%, respectively) after 82 h fermentations. A high initial ethanol concentration and moderate levels of glycerol and acetic acid accompanied glucose as co-substrate, whereas the ethanol concentration gradually increased with raffinose as co-substrate with no glycerol and much less acetic acid formation.lld:pubmed
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pubmed-article:10616716pubmed:authorpubmed-author:van ZylW HWHlld:pubmed
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pubmed-article:10616716pubmed:pagination829-33lld:pubmed
pubmed-article:10616716pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:10616716pubmed:articleTitleXylose utilisation by recombinant strains of Saccharomyces cerevisiae on different carbon sources.lld:pubmed
pubmed-article:10616716pubmed:affiliationDepartment of Microbiology, University of Stellenbosch, South Africa. WHVZ@maties.sun.ac.zalld:pubmed
pubmed-article:10616716pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10616716pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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