Linked Life Data

10610027

Source:http://linkedlifedata.com/resource/pubmed/id/10610027

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-1-31
pubmed:databankReference
pubmed:abstractText
We isolated two striated muscle actin genes from medaka Oryzias latipes. OIMA1 is a skeletal muscle actin gene expressed in somitic muscle and head muscle and OIMA2 is probably a cardiac muscle actin gene expressed in both somitic and cardiac muscle. The differential transcription mechanisms for these two genes were examined in embryos by introducing fusion genes in which the OIMA1 or OIMA2 upstream region was connected to the green fluorescent protein gene. Embryos were injected with these fusion genes at the 2-cell stage. A fusion gene containing the region up to -949 of OIMA1 exhibited strong expression in somitic muscle. The coexistence of two regions, -949/-662 and -421/-201, is necessary for skeletal muscle-specific expression of OIMA1. Two E boxes and other unidentified sequences cooperatively function to achieve the full activity of the enhancer -949/-662. As for OIMA2, the region up to -520 is sufficient for strong muscle-specific expression. The region between -520 and -174 of OIMA2 is necessary for specific expression in both skeletal and cardiac muscles. In addition to the CArG box located at -140, an E-box at -430 is important for the expression in cardiac muscle as well as skeletal muscle. When the enhancers for the two muscle actin genes were switched and combined with each other's promoter, they were able to upregulate tissue-specific expression according to their origin. These results suggest that distinct expression patterns of OIMA land OIMA2 are regulated by combination of regulatory modules, each of which contains multiple regulatory elements.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0214-6282
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
541-54
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10610027-Actins, pubmed-meshheading:10610027-Amino Acid Sequence, pubmed-meshheading:10610027-Animals, pubmed-meshheading:10610027-Base Sequence, pubmed-meshheading:10610027-DNA, Complementary, pubmed-meshheading:10610027-Embryo, Nonmammalian, pubmed-meshheading:10610027-Gene Expression Regulation, Developmental, pubmed-meshheading:10610027-Green Fluorescent Proteins, pubmed-meshheading:10610027-In Situ Hybridization, pubmed-meshheading:10610027-Luminescent Proteins, pubmed-meshheading:10610027-Molecular Sequence Data, pubmed-meshheading:10610027-Muscle, Skeletal, pubmed-meshheading:10610027-Myocardium, pubmed-meshheading:10610027-Oryzias, pubmed-meshheading:10610027-Ovum, pubmed-meshheading:10610027-Phylogeny, pubmed-meshheading:10610027-Promoter Regions, Genetic, pubmed-meshheading:10610027-Recombinant Fusion Proteins, pubmed-meshheading:10610027-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:10610027-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10610027-Somites
pubmed:year
1999
pubmed:articleTitle
In vivo analysis of two striated muscle actin promoters reveals combinations of multiple regulatory modules required for skeletal and cardiac muscle-specific gene expression.
pubmed:affiliation
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan. rie@bio.sci.hokudai.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't