Linked Life Data

10610022

Source:http://linkedlifedata.com/resource/pubmed/id/10610022

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-1-31
pubmed:abstractText
In developing limb bud, mesenchymal cells form cellular aggregates called "mesenchymal condensations". These condensations show the prepattern of skeletal elements of the limb prior to cartilage differentiation. Roles of various signaling molecules in chondrogenesis in the limb bud have been reported. One group of signaling factors includes the Wnt proteins, which have been shown to have an inhibitory effect on chondrogenesis in the limb bud. Therefore, regulation of Wnt activity may be important in regulating cartilage differentiation. Here we show that Frzb-1, which encodes a secreted frizzled-related protein that can bind to Wnt proteins and can antagonize the activity of some Wnts, is expressed in the developing limb bud. At early stages of limb development, Frzb-1 is expressed in the ventral core mesenchyme of the limb bud, and later Frzb-1 expression becomes restricted to the central core region where mesenchymal condensations occur. At these stages, a chondrogenic marker gene, aggrecan, is not yet expressed. As limb development proceeds, expression of Frzb-1 is detected in cartilage primordial cells, although ultimately Frzb-1 expression is down-regulated. Similar results were obtained in the recombinant limb bud, which was constructed from dissociated and re-aggregated mesenchymal cells and an ectodermal jacket with the apical ectodermal ridge. In addition, Frzb-1 expression preceded aggrecan expression in micromass cultures. These results suggest that Frzb-1 has a role in condensation formation and cartilage differentiation by regulating Wnt activity in the limb bud.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0214-6282
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
495-500
pubmed:dateRevised
2009-9-29
pubmed:meshHeading
pubmed-meshheading:10610022-Aggrecans, pubmed-meshheading:10610022-Animals, pubmed-meshheading:10610022-Cartilage, pubmed-meshheading:10610022-Cells, Cultured, pubmed-meshheading:10610022-Chick Embryo, pubmed-meshheading:10610022-Chondrogenesis, pubmed-meshheading:10610022-Extracellular Matrix Proteins, pubmed-meshheading:10610022-Gene Expression Regulation, Developmental, pubmed-meshheading:10610022-Glycoproteins, pubmed-meshheading:10610022-In Situ Hybridization, pubmed-meshheading:10610022-Lectins, C-Type, pubmed-meshheading:10610022-Limb Buds, pubmed-meshheading:10610022-Mesoderm, pubmed-meshheading:10610022-Microscopy, Phase-Contrast, pubmed-meshheading:10610022-Proteins, pubmed-meshheading:10610022-Proteoglycans, pubmed-meshheading:10610022-Proto-Oncogene Proteins, pubmed-meshheading:10610022-Wnt Proteins, pubmed-meshheading:10610022-Zebrafish Proteins
pubmed:year
1999
pubmed:articleTitle
Involvement of Frzb-1 in mesenchymal condensation and cartilage differentiation in the chick limb bud.
pubmed:affiliation
Department of Molecular Biology, Kawasaki Medical School, Kurashiki, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't