Source:http://linkedlifedata.com/resource/pubmed/id/10609875
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2-3
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| pubmed:dateCreated |
2000-2-1
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| pubmed:abstractText |
Macrophage migration inhibitory factor (MIF), a putative cytokine involved in inflammatory and immune responses, was identified in rat peritoneal macrophages by Western blot analysis and its secretion into culture medium by enzyme-linked immunosorbent assay. To clarify the possibility of vitamin E as an immune modulator, we investigated the effect of vitamin E on MIF production in macrophages in response to calcium ionophore A23187 and lipopolysaccharide (LPS). Intraperitoneal injections of vitamin E (5 mg per rat) for 6 successive days resulted in a significant increase of alpha-tocopherol content in peritoneal macrophages. Alpha-tocopherol content of macrophages in vitamin E-treated rats was 478.3 +/- 90.7 ng/10(6) cells, whereas in control rats it was 1.5 +/- 0.5 ng/10(6) cells. For the control macrophages, total MIF content of the medium (2.5 x 10(6) cells/18 ml) without stimulation was 40.7 +/- 3.6 ng after 14 h culture, whereas stimulation with calcium ionophore A23187 (400 nM) and LPS (5.0 microg/ml) induced the elevation of MIF content to 65.9 +/- 7.5 ng and 74.3 +/- 10.4 ng, respectively (p < 0.05, n = 3). On the other hand, vitamin E-enriched macrophages without stimulation showed less MIF content (14.0 +/- 4.2 ng) than the control (p < 0.05, n = 3). Similarly, the increase of MIF of vitamin E-treated macrophages was significantly suppressed after stimulation with calcium ionophore A23187 or LPS, compared with the control macrophages (p < 0.01, n = 3). From analysis of intracellular MIF content by Western blot, we found no alteration of intracellular MIF content of vitamin E-macrophages, in contrast to the decreased content of control stimulated-macrophages, showing that vitamin E suppressed MIF secretion into the culture medium. Taken together, these results indicate that vitamin E may contribute to the regulation of inflammatory and immune responses through regulation of MIF secretion, possibly by modulating macrophage-membrane architecture.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
0951-6433
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
10
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
139-43
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10609875-Animals,
pubmed-meshheading:10609875-Calcimycin,
pubmed-meshheading:10609875-Cell Size,
pubmed-meshheading:10609875-Cell Survival,
pubmed-meshheading:10609875-Cells, Cultured,
pubmed-meshheading:10609875-Kinetics,
pubmed-meshheading:10609875-Lipopolysaccharides,
pubmed-meshheading:10609875-Macrophage Migration-Inhibitory Factors,
pubmed-meshheading:10609875-Macrophages, Peritoneal,
pubmed-meshheading:10609875-Male,
pubmed-meshheading:10609875-Rats,
pubmed-meshheading:10609875-Rats, Wistar,
pubmed-meshheading:10609875-Vitamin E
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| pubmed:year |
1999
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| pubmed:articleTitle |
Effect of vitamin E on production of macrophage migration inhibitory factor (MIF) by macrophages.
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| pubmed:affiliation |
Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan. sakamoto@den.hokudai.ac.jp
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| pubmed:publicationType |
Journal Article
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