Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2000-2-3
pubmed:abstractText
1. The endothelial modulation of the relaxant responses to the nitric oxide (NO) donor sodium nitroprusside (SNP) and the KATP channel opener levcromakalim (LEM) and the interactions between these agents were analysed in isolated rat aorta. 2. LEM-induced relaxation was unchanged by endothelium removal or by the presence of L-NAME (10-4 M) or ODQ (10-6 M). In contrast, in KCl- (25 mM), but not in noradrenaline- (NA, 10-6 M) contracted arteries, SNP-induced relaxation was augmented by endothelium removal but not by L-NAME, indomethacin, glibenclamide nor charybdotoxin plus apamin. 3. The isobolographic analysis of the interactions between exogenously activated KATP channels and cyclic GMP using mixtures of SNP and LEM revealed that there were no interactions between both drugs at the proportions at which both drugs were active. However, the points for the SNP : LEM mixtures in proportions 10:1 and 1:10,000 (i.e. at concentrations at which LEM and SNP were inactive, respectively) fell significantly above the line of additivity indicating that there were negative interactions between both drugs at these selected proportions (about 5- and 2 fold inhibition, respectively). The former interaction was sensitive to glibenclamide, whereas the latter was insensitive ODQ. The magnitude of the 10:1 SNP:LEM interaction was smaller in endothelium-intact arteries and was absent in arteries stimulated by NA. 4. In conclusion, the relaxations induced by LEM and SNP were additive. However, the presence of endothelium and low concentrations of LEM inhibited SNP-induced relaxation. Both inhibitory effects were not additive and were only observed in KCl- and not in NA-contracted aortae.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-10193784, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-1469622, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-1797333, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-1852778, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-2495353, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-2580134, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-2677570, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-2851359, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-3263652, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-4043223, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-7521260, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-7712018, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-7733230, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-7913615, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-8298804, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-8358541, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-8494353, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-8571354, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-8581285, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-8851502, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-8894979, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-8945685, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-8989149, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-9042595, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-9113362, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-9535012, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-9650826, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-9692786, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-9720794, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-9788772, http://linkedlifedata.com/resource/pubmed/commentcorrection/10602320-9834033
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
128
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1419-26
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Vasodilator effects of sodium nitroprusside, levcromakalim and their combination in isolated rat aorta.
pubmed:affiliation
Departamento de Farmacología, Instituto de Farmacología y Toxicología (CSIC), Facultad de Medicina, Universidad Complutense de Madrid, 28040 Madrid, Spain. fperez@eucmax.sim.ucm.es
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't