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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2000-1-28
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pubmed:abstractText |
We have established a method for real-time video analysis of the interaction of antigen-presenting cells (APCs) with T cells. Green fluorescent protein expression controlled by a nuclear factor of activated T cells (NFAT)-responsive promoter permits the visualization of productive antigen presentation in single T cells. The readout is rapid (within 2 h) and semiquantitative and allows analysis by video microscopy and flow cytometry. Using this approach, we demonstrate that macrophages have the capacity to simultaneously activate multiple T cells. In addition, the interaction of T cells with macrophages is extraordinarily dynamic: after initial stable contact, the T cells migrate continuously on the surface of the macrophage and from APC to APC during productive antigen presentation. Thus, T cells sum up signals from multiple interactions with macrophages during stimulation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-10358769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-10398592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-1656278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-1902576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-2123468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-2394747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-2523714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-2530300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-2953845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-3124107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-3260003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-3526350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-7697352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-8985252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-9108078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-9133415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-9597140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-9738502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-9741631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-9856952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-9924026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-9988658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-9989490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601366-9989492
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
190
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1909-14
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1999
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pubmed:articleTitle |
Dynamic interactions of macrophages with T cells during antigen presentation.
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pubmed:affiliation |
Department of Immunology, University of Washington, Seattle, Washington 98195, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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