| pubmed-article:10600468 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10600468 | lifeskim:mentions | umls-concept:C1325725 | lld:lifeskim |
| pubmed-article:10600468 | lifeskim:mentions | umls-concept:C1093046 | lld:lifeskim |
| pubmed-article:10600468 | lifeskim:mentions | umls-concept:C0036025 | lld:lifeskim |
| pubmed-article:10600468 | lifeskim:mentions | umls-concept:C1136102 | lld:lifeskim |
| pubmed-article:10600468 | lifeskim:mentions | umls-concept:C0205164 | lld:lifeskim |
| pubmed-article:10600468 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:10600468 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:10600468 | pubmed:dateCreated | 2000-1-31 | lld:pubmed |
| pubmed-article:10600468 | pubmed:abstractText | Recombinant major capsid protein, L1 (M(r) = 55,000), of human papillomavirus type 11 was expressed intracellularly at high levels in a galactose-inducible Saccharomyces cerevisiae expression system by an HPV6/11 hybrid gene. The capsid protein self-assembled into virus-like particles (VLPs) and accounted for 15% of the total soluble protein. A purification process was developed that consisted of two main steps: microfiltration and cation-exchange chromatography. The purified VLPs were 98% homogeneous, and the overall purification yield was 10%. The final product was characterized by several analytical methods and was highly immunogenic in mice. | lld:pubmed |
| pubmed-article:10600468 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10600468 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10600468 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10600468 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10600468 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10600468 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10600468 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10600468 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10600468 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10600468 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10600468 | pubmed:issn | 1046-5928 | lld:pubmed |
| pubmed-article:10600468 | pubmed:author | pubmed-author:YipY KYK | lld:pubmed |
| pubmed-article:10600468 | pubmed:author | pubmed-author:KellerP MPM | lld:pubmed |
| pubmed-article:10600468 | pubmed:author | pubmed-author:LehmanE DED | lld:pubmed |
| pubmed-article:10600468 | pubmed:author | pubmed-author:GeorgeH AHA | lld:pubmed |
| pubmed-article:10600468 | pubmed:author | pubmed-author:SchultzL DLD | lld:pubmed |
| pubmed-article:10600468 | pubmed:author | pubmed-author:LoweR SRS | lld:pubmed |
| pubmed-article:10600468 | pubmed:author | pubmed-author:JansenK UKU | lld:pubmed |
| pubmed-article:10600468 | pubmed:author | pubmed-author:JoyceJ GJG | lld:pubmed |
| pubmed-article:10600468 | pubmed:author | pubmed-author:CookJ CJC | lld:pubmed |
| pubmed-article:10600468 | pubmed:author | pubmed-author:HurniW MWM | lld:pubmed |
| pubmed-article:10600468 | pubmed:author | pubmed-author:HeplerR WRW | lld:pubmed |
| pubmed-article:10600468 | pubmed:copyrightInfo | Copyright 1999 Academic Press. | lld:pubmed |
| pubmed-article:10600468 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10600468 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:10600468 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10600468 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10600468 | pubmed:pagination | 477-84 | lld:pubmed |
| pubmed-article:10600468 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:10600468 | pubmed:meshHeading | pubmed-meshheading:10600468... | lld:pubmed |
| pubmed-article:10600468 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10600468 | pubmed:articleTitle | Purification of virus-like particles of recombinant human papillomavirus type 11 major capsid protein L1 from Saccharomyces cerevisiae. | lld:pubmed |
| pubmed-article:10600468 | pubmed:affiliation | Department of Virus and Cell Biology, Merck Research Laboratories, West Point, Pennsylvania 19486, USA. | lld:pubmed |
| pubmed-article:10600468 | pubmed:publicationType | Journal Article | lld:pubmed |
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