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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-1-31
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pubmed:abstractText |
Recombinant major capsid protein, L1 (M(r) = 55,000), of human papillomavirus type 11 was expressed intracellularly at high levels in a galactose-inducible Saccharomyces cerevisiae expression system by an HPV6/11 hybrid gene. The capsid protein self-assembled into virus-like particles (VLPs) and accounted for 15% of the total soluble protein. A purification process was developed that consisted of two main steps: microfiltration and cation-exchange chromatography. The purified VLPs were 98% homogeneous, and the overall purification yield was 10%. The final product was characterized by several analytical methods and was highly immunogenic in mice.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1046-5928
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pubmed:author |
pubmed-author:CookJ CJC,
pubmed-author:GeorgeH AHA,
pubmed-author:HeplerR WRW,
pubmed-author:HurniW MWM,
pubmed-author:JansenK UKU,
pubmed-author:JoyceJ GJG,
pubmed-author:KellerP MPM,
pubmed-author:LehmanE DED,
pubmed-author:LoweR SRS,
pubmed-author:SchultzL DLD,
pubmed-author:YipY KYK
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
477-84
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10600468-Amino Acids,
pubmed-meshheading:10600468-Animals,
pubmed-meshheading:10600468-Antibody Formation,
pubmed-meshheading:10600468-Blotting, Western,
pubmed-meshheading:10600468-Capsid,
pubmed-meshheading:10600468-Capsid Proteins,
pubmed-meshheading:10600468-Chromatography, Ion Exchange,
pubmed-meshheading:10600468-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10600468-Humans,
pubmed-meshheading:10600468-Mice,
pubmed-meshheading:10600468-Mice, Inbred BALB C,
pubmed-meshheading:10600468-Microscopy, Electron,
pubmed-meshheading:10600468-Oncogene Proteins, Viral,
pubmed-meshheading:10600468-Papillomaviridae,
pubmed-meshheading:10600468-Recombinant Proteins,
pubmed-meshheading:10600468-Saccharomyces cerevisiae,
pubmed-meshheading:10600468-Virus Assembly
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pubmed:year |
1999
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pubmed:articleTitle |
Purification of virus-like particles of recombinant human papillomavirus type 11 major capsid protein L1 from Saccharomyces cerevisiae.
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pubmed:affiliation |
Department of Virus and Cell Biology, Merck Research Laboratories, West Point, Pennsylvania 19486, USA.
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pubmed:publicationType |
Journal Article
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