Linked Life Data

10595319

Source:http://linkedlifedata.com/resource/pubmed/id/10595319

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2000-1-11
pubmed:abstractText
Characteristics of dipeptide transport in pig jejunum were investigated in vitro by applying the Ussing-chamber technique and mucosal uptake studies. Addition of both glycyl-L-glutamine and glycyl-L-sarcosine (20 mmol.l-1) to the mucosal buffer solution significantly increased the short-circuit current by 2.60 +/- 0.15 and 1.57 +/- 0.20 mu eq.cm-2.h-1, respectively. Concentration-dependent changes in short-circuit current followed Michaelis-Menten kinetics with similar affinity constants for both dipeptides. From unidirectional flux rates for radiolabelled glycyl-L-sarcosine, a net flux rate for glycyl-L-sarcosine of 49.8 +/- 6.7 nmol.cm-2.h-1 was calculated. In mucosal uptake experiments, the apical influx of 14C-labelled glycyl-L-sarcosine into isolated porcine mucosa was pH dependent and significantly inhibited by glycyl-L-glutamine. Moreover, RT-PCR studies with primers derived from rabbit PepT1 identified two PCR fragments of identical size to rabbit PepT1 from pig intestinal mRNA preparations. In conclusion, our studies revealed key features of mammalian intestinal peptide transporters and give evidence for a PepT1-like transporter in the pig jejunum that could significantly contribute to the overall amino acid absorption from the gut.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0174-1578
pubmed:author
pubmed:issnType
Print
pubmed:volume
169
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
495-500
pubmed:dateRevised
2009-6-8
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Characteristics of dipeptide transport in pig jejunum in vitro.
pubmed:affiliation
Research Centre for Animal Production and Technology, Georg-August University of Göttingen, Germany.
pubmed:publicationType
Journal Article, In Vitro