Linked Life Data

10589972

Source:http://linkedlifedata.com/resource/pubmed/id/10589972

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-1-11
pubmed:abstractText
The present study focuses on the role of endothelium on alpha1-adrenoceptor-mediated vasoconstriction in aorta from Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR). To define and quantify changes in the parameters governing agonism at alpha1-adrenoceptor by hypertension and/or endothelium, the operational model of analysis was used. In either endothelium intact or denuded aorta, the sensitivity (P < 0.001) and the maximum contraction (P < 0.05) to phenylephrine were smaller in SHR than in WKY. However, in each strain of rats, removal of endothelium increased the sensitivity (P < 0.001) to phenylephrine but reduced (P < 0.05) KCl-evoked vasoconstriction, suggesting a modulation of these responses by the endothelium. The observed differences in sensitivity and maximum contraction are interpreted in terms of the operational parameters: Em, the maximum possible effect; pK(A), the agonist affinity; alpha, the agonist efficacy and n, the slope for the function relating receptor occupancy to pharmacological effect. The estimated parameters reflected differences, between strains, in the signal transduction processes linked to alpha1-adrenoceptor stimulation ascribed to the presence of the endothelium. N(G)-nitro-L-arginine methyl ester (L-NAME), enhanced to a similar extend in both rat strains the sensitivity (P < 0.001) and the maximum contraction to phenylephrine. Indomethacin reduced the maximum contraction to phenylephrine by 56.85% in SHR and by 11.80% in WKY suggesting that contracting prostanoids play a more major role in this response in SHR than in WKY. Nevertheless, these inhibitors were without effect on denuded vessels from both strains suggesting that NO and cyclooxygenase products from the media or the adventitia do not play a role on the phenylephrine-mediated responses. The studied endothelial factors partially explain the observed differences in modulation of alpha1-adrenoceptor responses by the endothelium but suggest the participation of other compounds released by the endothelium.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0144-1795
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
219-28
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10589972-Adrenergic alpha-Agonists, pubmed-meshheading:10589972-Algorithms, pubmed-meshheading:10589972-Animals, pubmed-meshheading:10589972-Aorta, Thoracic, pubmed-meshheading:10589972-Carotid Arteries, pubmed-meshheading:10589972-Endothelium, Vascular, pubmed-meshheading:10589972-Enzyme Inhibitors, pubmed-meshheading:10589972-Hypertension, pubmed-meshheading:10589972-Models, Biological, pubmed-meshheading:10589972-NG-Nitroarginine Methyl Ester, pubmed-meshheading:10589972-Nitric Oxide, pubmed-meshheading:10589972-Nitric Oxide Synthase, pubmed-meshheading:10589972-Nitric Oxide Synthase Type III, pubmed-meshheading:10589972-Phenylephrine, pubmed-meshheading:10589972-Rats, pubmed-meshheading:10589972-Rats, Inbred SHR, pubmed-meshheading:10589972-Rats, Inbred WKY, pubmed-meshheading:10589972-Receptors, Adrenergic, alpha, pubmed-meshheading:10589972-Receptors, Adrenergic, alpha-1, pubmed-meshheading:10589972-Vasoconstriction
pubmed:year
1999
pubmed:articleTitle
Modelling the changes due to the endothelium and hypertension in the alpha-adrenoreceptor-mediated responses of rat aorta.
pubmed:affiliation
Departament de Farmacologia i Terapèutica, Unitat de Bioestadística, Facultat de Medicina, Universitat Autònoma de Barcelona, Spain.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't