Linked Life Data

10587472

Source:http://linkedlifedata.com/resource/pubmed/id/10587472

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pubmed-article:10587472pubmed:abstractTextA total of 1000 expressed sequence tags (ESTs) corresponding to 760 unique sequence sets were identified using random sequencing of clones from a cDNA library constructed from mycelial RNA of Phytophthora infestans. A number of software programs, represented by a relational database and an analysis pipeline, were developed for the automated analysis and storage of the EST sequence data. A set of 419 nonredundant sequences, which correspond to a total of 632 ESTs (63.2%), were identified as showing significant matches to sequences deposited in public databases. A putative cellular identity and role was assigned to all 419 sequences. All major functional categories were represented by at least several ESTs. Four novel cDNAs containing sequences related to elicitins, a family of structurally related proteins that induce the hypersensitive response and condition avirulence of P. infestans on Nicotiana plants, were among the most notable genes identified. Two of these elicitin-like cDNAs were among the most abundant cDNAs examined. The set also contained several ESTs with high sequence similarity to unique plant genes.lld:pubmed
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pubmed-article:10587472pubmed:copyrightInfoCopyright 1999 Academic Press.lld:pubmed
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pubmed-article:10587472pubmed:dateRevised2008-11-21lld:pubmed
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pubmed-article:10587472pubmed:articleTitleInitial assessment of gene diversity for the oomycete pathogen Phytophthora infestans based on expressed sequences.lld:pubmed
pubmed-article:10587472pubmed:affiliationDepartment of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, 1680 Madison Avenue, Wooster, Ohio 44691, USA.lld:pubmed
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